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Two tomato GDP-D-mannose epimerase isoforms involved in ascorbate biosynthesis play specific roles in cell wall biosynthesis and development

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMOUNET-GILBERT, Louise
hal.structure.identifierLaboratoire de Glycobiologie et Matrice Extracellulaire Végétale [Glyco-MEV]
dc.contributor.authorDUMONT, Marie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERRAND, Carine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBOURNONVILLE, Celine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMONIER, Antoine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJORLY, Joana
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLEMAIRE-CHAMLEY, Martine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMORI, Kentaro
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorATIENZA, Isabelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorHERNOULD, Michel
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorSTEVENS, Rebecca
hal.structure.identifierLaboratoire de Glycobiologie et Matrice Extracellulaire Végétale [Glyco-MEV]
dc.contributor.authorLEHNER, Arnaud
hal.structure.identifierLaboratoire de Glycobiologie et Matrice Extracellulaire Végétale [Glyco-MEV]
dc.contributor.authorMOLLET, Jean Claude
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierLaboratoire de Glycobiologie et Matrice Extracellulaire Végétale [Glyco-MEV]
dc.contributor.authorLEROUGE, Patrice
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
dc.date.issued2016
dc.identifier.issn0022-0957
dc.description.abstractEnGDP-D-mannose epimerase (GME, EC 5.1.3.18) converts GDP-D-mannose to GDP-L-galactose, and is considered to be a central enzyme connecting the major ascorbate biosynthesis pathway to primary cell wall metabolism in higher plants. Our previous work demonstrated that GME is crucial for both ascorbate and cell wall biosynthesis in tomato. The aim of the present study was to investigate the respective role in ascorbate and cell wall biosynthesis of the two SlGME genes present in tomato by targeting each of them through an RNAi-silencing approach. Taken individually SlGME1 and SlGME2 allowed normal ascorbate accumulation in the leaf and fruits, thus suggesting the same function regarding ascorbate. However, SlGME1 and SlGME2 were shown to play distinct roles in cell wall biosynthesis, depending on the tissue considered. The RNAi-SlGME1 plants harbored small and poorly seeded fruits resulting from alterations of pollen development and of pollination process. In contrast, the RNAi-SlGME2 plants exhibited vegetative growth delay while fruits remained unaffected. Analysis of SlGME1-and SlGME2-silenced seeds and seedlings further showed that the dimerization state of pectin rhamnogalacturonan-II (RG-II) was altered only in the RNAi-SlGME2 lines. Taken together with the preferential expression of each SlGME gene in different tomato tissues, these results suggest sub-functionalization of SlGME1 and SlGME2 and their specialization for cell wall biosynthesis in specific tomato tissues.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enGDP-D-mannose epimerase
dc.subject.encell wall
dc.subject.entomato fruits
dc.subject.enSolanum lycopersicum
dc.subject.enrhamnogalacturonan-II
dc.subject.engrowth
dc.subject.enAscorbate
dc.subject.enAsA: ascorbic acid
dc.subject.enDPA: days post anthesis
dc.subject.enPMI: phosphomannose isomerase
dc.subject.enGMP: GDP-D-mannose pyrophosphorylase
dc.subject.enGME: GDP-D-mannose-3
dc.subject.en5-epimerase
dc.title.enTwo tomato GDP-D-mannose epimerase isoforms involved in ascorbate biosynthesis play specific roles in cell wall biosynthesis and development
dc.typeArticle de revue
dc.identifier.doi10.1093/jxb/erw260
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Botanique
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalJournal of Experimental Botany
bordeaux.page4767-4777
bordeaux.volume67
bordeaux.issue15
bordeaux.peerReviewedoui
hal.identifierhal-01775273
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01775273v1
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