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hal.structure.identifierPlant Environmental Physiology and Stress Signaling [PEPSS]
dc.contributor.authorBONNOT, Clémence
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorPINSON, Benoît
hal.structure.identifierPlant Environmental Physiology and Stress Signaling [PEPSS]
dc.contributor.authorCLÉMENT, Mathilde
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBERNILLON, Stéphane
hal.structure.identifierPlant Environmental Physiology and Stress Signaling [PEPSS]
dc.contributor.authorCHIARENZA, Serge
hal.structure.identifierPlant Environmental Physiology and Stress Signaling [PEPSS]
dc.contributor.authorKANNO, Satomi
hal.structure.identifierTokyo University of Science [Tokyo]
hal.structure.identifierGraduate School of Agricultural and Life Sciences [UTokyo] [GSALS]
dc.contributor.authorKOBAYASHI, Natsuko I.
hal.structure.identifierPlant Environmental Physiology and Stress Signaling [PEPSS]
dc.contributor.authorDELANNOY, Etienne
hal.structure.identifierGraduate School of Agricultural and Life Sciences [UTokyo] [GSALS]
dc.contributor.authorNAKANISHI, Tomoko M
hal.structure.identifierPlant Environmental Physiology and Stress Signaling [PEPSS]
dc.contributor.authorNUSSAUME, Laurent
hal.structure.identifierPlant Environmental Physiology and Stress Signaling [PEPSS]
dc.contributor.authorDESNOS, Thierry
dc.date.issued2016-01
dc.identifier.issn0028-646X
dc.description.abstractEnPlants display numerous strategies to cope with phosphate (Pi)-deficiency. Despite multiple genetic studies, the molecular mechanisms of low-Pi-signalling remain unknown. To validate the interest of chemical genetics to investigate this pathway we discovered and analysed the effects of PHOSTIN (PSN), a drug mimicking Pi-starvation in Arabidopsis. We assessed the effects of PSN and structural analogues on the induction of Pi-deficiency responses in mutants and wild-type and followed their accumulation in plants organs by high pressure liquid chromotography (HPLC) or mass-spectrophotometry. We show that PSN is cleaved in the growth medium, releasing its active motif (PSN11), which accumulates in plants roots. Despite the overaccumulation of Pi in the roots of treated plants, PSN11 elicits both local and systemic Pi-starvation effects. Nevertheless, albeit that the transcriptional activation of low-Pi genes by PSN11 is lost in the phr1;phl1 double mutant, neither PHO1 nor PHO2 are required for PSN11 effects. The range of local and systemic responses to Pi-starvation elicited, and their dependence on the PHR1/PHL1 function suggests that PSN11 affects an important and early step of Pi-starvation signalling. Its independence from PHO1 and PHO2 suggest the existence of unknown pathway(s), showing the usefulness of PSN and chemical genetics to bring new elements to this field.
dc.description.sponsorshipGénétique d'une voie limitant la croissance racinaire d'Arabidopsis en réponse à la carence phosphatée
dc.description.sponsorshipPhosphate signalling - ANR-08-KBBE-0006
dc.language.isoen
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectchemical genetics
dc.subject.enArabidopsis thaliana
dc.subject.enOryza sativa
dc.subject.enPHOSTIN
dc.subject.enphosphate homeostasis
dc.subject.enphosphate starvation
dc.subject.enphr1
dc.subject.enphl1 mutant
dc.title.enA chemical genetic strategy identify the PHOSTIN, a synthetic molecule that triggers phosphate starvation responses in Arabidopsis thaliana
dc.typeArticle de revue
dc.identifier.doi10.1111/nph.13591
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalNew Phytologist
bordeaux.page161-176
bordeaux.volume209
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifiercea-02143828
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//cea-02143828v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=New%20Phytologist&rft.date=2016-01&rft.volume=209&rft.issue=1&rft.spage=161-176&rft.epage=161-176&rft.eissn=0028-646X&rft.issn=0028-646X&rft.au=BONNOT,%20Cl%C3%A9mence&PINSON,%20Beno%C3%AEt&CL%C3%89MENT,%20Mathilde&BERNILLON,%20St%C3%A9phane&CHIARENZA,%20Serge&rft.genre=article


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