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The GMO90+ Project: Absence of Evidence for Biologically Meaningful Effects of Genetically Modified Maize-based Diets on Wistar Rats After 6-Months Feeding Comparative Trial

hal.structure.identifierToxicité environnementale, cibles thérapeutiques, signalisation cellulaire [T3S - UMR_S 1124]
dc.contributor.authorCOUMOUL, Xavier
hal.structure.identifierToxAlim [ToxAlim]
dc.contributor.authorSERVIEN, Rémi
hal.structure.identifierToxicité environnementale, cibles thérapeutiques, signalisation cellulaire [T3S - UMR_S 1124]
dc.contributor.authorJURICEK, Ludmila
hal.structure.identifierToxicité environnementale, cibles thérapeutiques, signalisation cellulaire [T3S - UMR_S 1124]
dc.contributor.authorKADDOUCH-AMAR, Yael
hal.structure.identifierTranscriptomic impact of Xenobiotics [E23 TRiX]
dc.contributor.authorLIPPI, Yannick
hal.structure.identifierCentre de Recherche en Transplantation et Immunologie [U1064 Inserm - CRTI]
dc.contributor.authorBERTHELOT, Laureline
hal.structure.identifierLaboratoire de Génétique Cellulaire [LGC]
dc.contributor.authorNAYLIES, Claire
hal.structure.identifierÉcole nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique [ONIRIS]
dc.contributor.authorMORVAN, Marie-Line
hal.structure.identifierÉcole nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique [ONIRIS]
dc.contributor.authorANTIGNAC, Jean-Philippe
hal.structure.identifierInstitut de recherche en santé, environnement et travail [Irset]
dc.contributor.authorDESDOITS-LETHIMONIER, Christèle
hal.structure.identifierGroupe d'Etude de la Reproduction Chez l'Homme et les Mammiferes [GERHM]
dc.contributor.authorJÉGOU, Bernard
hal.structure.identifierXénobiotiques
dc.contributor.authorTREMBLAY-FRANCO, Marie
hal.structure.identifierMetaToul AXIOM [E20]
dc.contributor.authorCANLET, Cécile
hal.structure.identifierMetaToul AXIOM [E20]
dc.contributor.authorDEBRAUWER, Laurent
hal.structure.identifierMethodOmics [Toulouse] [Recherche-Développement en Biotechnologie]
dc.contributor.authorLE GALL, Caroline
dc.contributor.authorLAURENT, Julie
hal.structure.identifierMethodomics
dc.contributor.authorGOURAUD, Pierre-Antoine
hal.structure.identifierXénobiotiques
dc.contributor.authorCRAVEDI, Jean Pierre
hal.structure.identifierToxAlim [ToxAlim]
dc.contributor.authorJEUNESSE, Élisabeth
dc.contributor.authorSAVY, Nicolas
hal.structure.identifierToxAlim [ToxAlim]
dc.contributor.authorDANDERE-ABDOULKARIM, Kadidiatou
hal.structure.identifierDirection de l'Evaluation des Risques [DER]
dc.contributor.authorARNICH, Nathalie
hal.structure.identifierDirection de l'Evaluation des Risques [DER]
dc.contributor.authorFOURÈS, Franck
hal.structure.identifierProfilomic [Boulogne-Billancourt]
dc.contributor.authorCOTTON, Jérôme
hal.structure.identifierProfilomic [Boulogne-Billancourt]
dc.contributor.authorBROUDIN, Simon
hal.structure.identifierProfilomic
dc.contributor.authorCORMAN, Bruno
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMOING, Annick
hal.structure.identifierToxAlim [ToxAlim]
dc.contributor.authorLAPORTE, Bérengère
hal.structure.identifierUnité de recherche Mycologie et Sécurité des Aliments [MycSA]
dc.contributor.authorRICHARD-FORGET, Florence
hal.structure.identifierHôpital Necker - Enfants Malades [AP-HP]
dc.contributor.authorBAROUKI, Robert
hal.structure.identifierReproduction et développement des plantes [RDP]
dc.contributor.authorROGOWSKY, Peter
hal.structure.identifierInstitut de pharmacologie et de biologie structurale [IPBS]
dc.contributor.authorSALLES, Bernard
dc.date.issued2019-04-01
dc.identifier.issn1096-6080
dc.description.abstractEnThe GMO90+ project was designed to identify biomarkers of exposure or health effects in Wistar Han RCC rats exposed in their diet to 2 genetically modified plants (GMP) and assess additional information with the use of metabolomic and transcriptomic techniques. Rats were fed for 6-months with 8 maize-based diets at 33% that comprised either MON810 (11% and 33%) or NK603 grains (11% and 33% with or without glyphosate treatment) or their corresponding near-isogenic controls. Extensive chemical and targeted analyses undertaken to assess each diet demonstrated that they could be used for the feeding trial. Rats were necropsied after 3 and 6 months. Based on the Organization for Economic Cooperation and Development test guideline 408, the parameters tested showed a limited number of significant differences in pairwise comparisons, very few concerning GMP versus non-GMP. In such cases, no biological relevance could be established owing to the absence of difference in biologically linked variables, dose-response effects, or clinical disorders. No alteration of the reproduction function and kidney physiology was found. Metabolomics analyses on fluids (blood, urine) were performed after 3, 4.5, and 6 months. Transcriptomics analyses on organs (liver, kidney) were performed after 3 and 6 months. Again, among the significant differences in pairwise comparisons, no GMP effect was observed in contrast to that of maize variety and culture site. Indeed, based on transcriptomic and metabolomic data, we could differentiate MON- to NK-based diets. In conclusion, using this experimental design, no biomarkers of adverse health effect could be attributed to the consumption of GMP diets in comparison with the consumption of their near-isogenic non-GMP controls.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subject.en6-month rat feeding trial
dc.subject.enTranscriptomics
dc.subject.enMetabolomics
dc.subject.enGenetically modified maize
dc.subject.enOECD TG408
dc.subject.enNK603
dc.subject.enMON810
dc.title.enThe GMO90+ Project: Absence of Evidence for Biologically Meaningful Effects of Genetically Modified Maize-based Diets on Wistar Rats After 6-Months Feeding Comparative Trial
dc.typeArticle de revue
dc.identifier.doi10.1093/toxsci/kfy298
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
dc.subject.halSciences du Vivant [q-bio]/Biologie cellulaire/Organisation et fonctions cellulaires [q-bio.SC]
dc.subject.halSciences du Vivant [q-bio]/Cancer
dc.subject.halSciences du Vivant [q-bio]/Ecologie, Environnement/Santé
dc.subject.halSciences du Vivant [q-bio]/Toxicologie
dc.subject.halSciences du Vivant [q-bio]/Toxicologie/Toxicologie et chaîne alimentaire
dc.subject.halSciences de l'environnement
dc.subject.halSciences de l'environnement/Environnement et Société
bordeaux.journalToxicological Sciences
bordeaux.page315-338
bordeaux.volume168
bordeaux.issue2
bordeaux.peerReviewedoui
hal.identifierhal-02196332
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02196332v1
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