Afficher la notice abrégée

Classical and next generation sequencing approaches unravel <em>Bymovirus</em> diversity in barley crops in France.

hal.structure.identifierGroupe d'Etude et de Contrôle des Variétés et des Semences [GEVES]
dc.contributor.authorROLLAND, Mathieu
hal.structure.identifierGroupe d'Etude et de Contrôle des Variétés et des Semences [GEVES]
dc.contributor.authorVILLEMOT, Julie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorTHEIL, Sébastien
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFAURE, Chantal
hal.structure.identifierGroupe d'Etude et de Contrôle des Variétés et des Semences [GEVES]
dc.contributor.authorCADOT, Valérie
hal.structure.identifierARVALIS - Institut du végétal [Paris]
dc.contributor.authorVALADE, Romain
hal.structure.identifierARVALIS - Institut du végétal [Paris]
dc.contributor.authorVITRY, Cindy
hal.structure.identifierJulius Kühn-Institut [JKI]
dc.contributor.authorRABENSTEIN, Frank
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.issued2017
dc.identifier.issn1932-6203
dc.description.abstractEnDespite the generalized use of cultivars carrying the rym4 resistance gene, the impact of viral mosaic diseases on winter barleys increased in recent years in France. This change could reflect i) an increased prevalence of the rym4 resistance-breaking pathotype of Barley yellow mosaic virus Y (BaYMV-2), ii) the emergence of rym4 resistance-breaking pathotypes of Barley mild mosaic virus (BaMMV) or iii) the emergence of other viruses. A study was undertaken to determine the distribution and diversity of viruses causing yellow mosaic disease. A collection of 241 symptomatic leaf samples from susceptible, rym4 and rym5 varieties was gathered from 117 sites. The viruses present in all samples were identified by specific RT-PCR assays and, for selected samples, by double-stranded RNA next-generation sequencing (NGS). The results show that BaYMV-2 is responsible for the symptoms observed in varieties carrying the resistance gene rym4. In susceptible varieties, both BaYMV-1 and BaYMV-2 were detected, together with BaMMV. Phylogenetic analyses indicate that the rym4 resistance-breaking ability independently evolved in multiple genetic backgrounds. Parallel analyses revealed a similar scenario of multiple independent emergence events in BaMMV for rym5 resistance-breaking, likely involving multiple amino acid positions in the viral-linked genome protein. NGS analyses and classical techniques provided highly convergent results, highlighting and validating the power of NGS approaches for diagnostics and viral population characterization.
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enClassical and next generation sequencing approaches unravel <em>Bymovirus</em> diversity in barley crops in France.
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0188495
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.journalPLoS ONE
bordeaux.pagee0188495
bordeaux.volume12
bordeaux.issue11
bordeaux.peerReviewedoui
hal.identifierhal-02620671
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02620671v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=PLoS%20ONE&amp;rft.date=2017&amp;rft.volume=12&amp;rft.issue=11&amp;rft.spage=e0188495&amp;rft.epage=e0188495&amp;rft.eissn=1932-6203&amp;rft.issn=1932-6203&amp;rft.au=ROLLAND,%20Mathieu&amp;VILLEMOT,%20Julie&amp;MARAIS,%20Armelle&amp;THEIL,%20S%C3%A9bastien&amp;FAURE,%20Chantal&amp;rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée