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Variable membrane protein A of flavescence dorée phytoplasma binds the midgut perimicrovillar membrane of <em>Euscelidius variegatus</em> and promotes adhesion to its epithelial cells

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARRICAU-BOUVERY, Nathalie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDURET, Sybille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDUBRANA, Marie-Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBATAILLER, Brigitte
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDESQUE, Delphine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDANET, Jean-Luc
hal.structure.identifierUniversità degli studi di Torino = University of Turin [UNITO]
dc.contributor.authorMONTICONE, Michela
hal.structure.identifierUniversità degli studi di Torino = University of Turin [UNITO]
dc.contributor.authorBOSCO, Domenico
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMALEMBIC-MAHER, Sylvie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFOISSAC, Xavier
dc.date.issued2018
dc.identifier.issn0099-2240
dc.description.abstractEnPhytoplasmas are uncultivated plant pathogens and cell wall-less bacteria and are transmitted from plant to plant by hemipteran insects. Phytoplasmas' circulative propagative cycle in insects requires the crossing of the midgut and salivary glands, and primary adhesion to cells is an initial step towards the invasion process. The flavescence dorée phytoplasma possesses a set of variable membrane proteins (Vmps) exposed to its surface, and this pathogen is suspected to interact with insect cells. The results showed that VmpA is expressed by the flavescence dorée phytoplasma present in the midgut and salivary glands. Phytoplasmas cannot be cultivated at present, and no mutant can be produced to investigate the putative role of Vmps in the adhesion of phytoplasma to insect cells. To overcome this difficulty, we engineered the Spiroplasma citri mutant G/6, which lacks the adhesins ScARPs, for VmpA expression and used VmpA-coated fluorescent beads to determine if VmpA acts as an adhesin in ex vivo adhesion assays and in vivo ingestion assays. VmpA specifically interacted with Euscelidius variegatus insect cells in culture and promoted the retention of VmpA-coated beads to the midgut of E. variegatus In this latest case, VmpA-coated fluorescent beads were localized and embedded in the perimicrovillar membrane of the insect midgut. Thus, VmpA functions as an adhesin that could be essential in the colonization of the insect by the FD phytoplasmas.Importance Phytoplasmas infect a wide variety of plants, ranging from wild plants to cultivated species, and are transmitted by different leafhoppers, planthoppers and psyllids. The specificity of the phytoplasma-insect vector interaction has a major impact on the phytoplasma plant host range. As entry into insect cells is an obligate process for phytoplasma transmission, the bacterial adhesion to insect cells is a key step. Thus, studying surface-exposed proteins of phytoplasma will help to identify the adhesins implicated in the specific recognition of insect vectors. In this study, it is shown that the membrane protein VmpA of the flavescence dorée phytoplasma acts as an adhesin that is able to interact with cells of Euscelidius variegatus, the experimental vector of the FD phytoplasma.
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.subject.enbacterial adhesion to cell
dc.subject.eninsect vector
dc.subject.enphytoplasma
dc.subject.entransmission
dc.title.enVariable membrane protein A of flavescence dorée phytoplasma binds the midgut perimicrovillar membrane of <em>Euscelidius variegatus</em> and promotes adhesion to its epithelial cells
dc.typeArticle de revue
dc.identifier.doi10.1128/AEM.02487-17
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalApplied and Environmental Microbiology
bordeaux.pagee02487-17
bordeaux.volume84
bordeaux.issue8
bordeaux.peerReviewedoui
hal.identifierhal-02621624
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02621624v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Applied%20and%20Environmental%20Microbiology&amp;rft.date=2018&amp;rft.volume=84&amp;rft.issue=8&amp;rft.spage=e02487-17&amp;rft.epage=e02487-17&amp;rft.eissn=0099-2240&amp;rft.issn=0099-2240&amp;rft.au=ARRICAU-BOUVERY,%20Nathalie&amp;DURET,%20Sybille&amp;DUBRANA,%20Marie-Pierre&amp;BATAILLER,%20Brigitte&amp;DESQUE,%20Delphine&amp;rft.genre=article


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