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High-Throughput Sequencing Reveals <em>Cyclamen persicum</em> Mill. as a Natural Host for Fig Mosaic Virus.

hal.structure.identifierCentre International de Hautes Etudes Agronomiques Méditerranéennes - Institut Agronomique Méditerranéen de Bari [CIHEAM-IAMB]
dc.contributor.authorELBEAINO, Toufic
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFAURE, Chantal
hal.structure.identifierNational Research Council of Italy | Consiglio Nazionale delle Ricerche [CNR]
dc.contributor.authorTRIOANO, Elisa
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierNational Research Council of Italy | Consiglio Nazionale delle Ricerche [CNR]
dc.contributor.authorPARRELLA, Giuseppe
dc.date.issued2018
dc.identifier.issn1999-4915
dc.description.abstractEnIn a search for viral infections, double-stranded RNA (dsRNA) were recovered from a diseased cyclamen (<em>Cyclamen persicum</em> Mill.) accession (Cic) and analyzed by high-throughput sequencing (HTS) technology. Analysis of the HTS data showed the presence of Fig mosaic emaravirus (FMV) in this accession. The complete sequences of six FMV-Cic RNA genomic segments were determined from the HTS data and using Sanger sequencing. All FMV-Cic RNA segments are similar in size to those of FMV from fig (FMV-Gr10), with the exception of RNA-6 that is one nucleotide longer. The occurrence of FMV in cyclamen was investigated through a small-scale survey, from which four plants (out of 18 tested) were found RT-PCR positive. To study sequence variations of cyclamen isolates of FMV, RT-PCR products generated through the amplification of the partially RNA-dependent RNA polymerase (RdRp, RNA-1), glycoprotein (GP, RNA-2), and nucleocapsid (NCP, RNA-3) genes were explored. The nucleotide sequence identities for cyclamen isolates ranged between 86% and 99% in RNA-1, 93% and 99% in RNA-2, and 98% and 99% in RNA-3, while lower identity levels were observed with the sequences of fig isolates. Based on the phylogenetic tree obtained with a 304-nt fragment of RNA3, all FMV isolates from cyclamens were assigned to a single cluster close to fig isolates from the Mediterranean. FMV was graft-transmitted to healthy cyclamens eliciting symptoms similar to those observed in the Cic accession, thus suggesting a causal role of FMV in the symptoms that prompted the investigation. This is the first report of FMV in a non-fig host, <em>Cyclamen persicum</em>, a finding that may help in the control of the mosaic and mosaic-like diseases of fig and cyclamen, respectively.
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectcyclamen
dc.subject.enFig mosaic emaravirus
dc.subject.enhigh-throughput sequencing
dc.title.enHigh-Throughput Sequencing Reveals <em>Cyclamen persicum</em> Mill. as a Natural Host for Fig Mosaic Virus.
dc.typeArticle de revue
dc.identifier.doi10.3390/v10120684
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.journalViruses
bordeaux.page684
bordeaux.volume10
bordeaux.issue12
bordeaux.peerReviewedoui
hal.identifierhal-02621862
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02621862v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Viruses&amp;rft.date=2018&amp;rft.volume=10&amp;rft.issue=12&amp;rft.spage=684&amp;rft.epage=684&amp;rft.eissn=1999-4915&amp;rft.issn=1999-4915&amp;rft.au=ELBEAINO,%20Toufic&amp;MARAIS,%20Armelle&amp;FAURE,%20Chantal&amp;TRIOANO,%20Elisa&amp;CANDRESSE,%20Thierry&amp;rft.genre=article


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