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Catastrophic Unbalanced Genome Rearrangements Cause Somatic Loss of Berry Color in Grapevine.

hal.structure.identifierUniversidad de La Rioja [UR]
dc.contributor.authorCARBONELL-BEJERANO, Pablo
hal.structure.identifierUniversidad de La Rioja [UR]
dc.contributor.authorROYO, Carolina
hal.structure.identifierUniversidad de La Rioja [UR]
dc.contributor.authorTORRES-PÉREZ, Rafael
hal.structure.identifierUniversidad de La Rioja [UR]
dc.contributor.authorGRIMPLET, Jérôme
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERNANDEZ, Lucie
hal.structure.identifierCentro Nacional de Biotecnología [Madrid] [CNB-CSIC]
dc.contributor.authorFRANCO-ZORRILLA, Jose Manuel
hal.structure.identifierConsejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] [CONICET]
dc.contributor.authorLIJAVETZKY, Diego
hal.structure.identifierUniversidad de La Rioja [UR]
dc.contributor.authorBAROJA, Elisa
hal.structure.identifierUniversidad de La Rioja [UR]
dc.contributor.authorMARTÍNEZ, Juana
hal.structure.identifierUniversidad de La Rioja [UR]
dc.contributor.authorGARCÍA-ESCUDERO, Enrique
hal.structure.identifierUniversidad de La Rioja [UR]
dc.contributor.authorIBANEZ, Javier
hal.structure.identifierUniversidad de La Rioja [UR]
dc.contributor.authorMARTINEZ-ZAPATER, José Miguel
dc.date.issued2017
dc.identifier.issn0032-0889
dc.description.abstractEnGrape (Vitis vinifera) color somatic variants that can be used to develop new grapevine cultivars occasionally appear associated with deletion events of uncertain origin. To understand the mutational mechanisms generating somatic structural variation in grapevine, we compared the Tempranillo Blanco (TB) white berry somatic variant with its black berry ancestor, Tempranillo Tinto. Whole-genome sequencing uncovered a catastrophic genome rearrangement in TB that caused the hemizygous deletion of 313 genes, including the loss of the functional copy for the MYB transcription factors required for anthocyanin pigmentation in the berry skin. Loss of heterozygosity and decreased copy number delimited interspersed monosomic and disomic regions in the right arm of linkage groups 2 and 5. At least 11 validated clustered breakpoints involving intrachromosomal and interchromosomal translocations between three linkage groups flanked the deleted fragments, which, according to segregation analyses, are phased in a single copy of each of the affected chromosomes. These hallmarks, along with the lack of homology between breakpoint joins and the randomness of the order and orientation of the rearranged fragments, are all consistent with a chromothripsis-like pattern generated after chromosome breakage and illegitimate rejoining. This unbalanced genome reshuffling has additional consequences in reproductive development. In TB, lack of sexual transmission of rearranged chromosomes associates with low gamete viability, which compromises fruit set and decreases fruit production. Our findings show that catastrophic genome rearrangements arise spontaneously and stabilize during plant somatic growth. These dramatic rearrangements generate new interesting phenotypes that can be selected for the improvement of vegetatively propagated plant species.
dc.language.isoen
dc.publisherOxford University Press ; American Society of Plant Biologists
dc.title.enCatastrophic Unbalanced Genome Rearrangements Cause Somatic Loss of Berry Color in Grapevine.
dc.typeArticle de revue
dc.identifier.doi10.1104/pp.17.00715
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant Physiology
bordeaux.page786-801
bordeaux.volume175
bordeaux.issue2
bordeaux.peerReviewedoui
hal.identifierhal-02626896
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02626896v1
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