Show simple item record

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMA, Yuxin
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLEFEBVRE, Marie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorTHEIL, Sébastien
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSVANELLA-DUMAS, Laurence
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFAURE, Chantal
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.issued2020
dc.identifier.issn0022-538X
dc.description.abstractEnThere are today significant knowledge gaps on phytovirus populations and on the drivers impacting them, but also on the comparative performance methodological approaches for their study. We used and compared two viral sequences enrichment approaches, double-stranded RNAs (dsRNA) and virion-associated nucleic acids (VANA) for phytovirome description in complex pools representative of the most prevalent plant species in unmanaged and cultivated ecosystems. Viral richness was assessed by determining Operational Taxonomic Units (OTU) following the clustering of conserved viral domains. There is some limited evidence of an impact of cultivation on viral populations. These results provide data allowing to reason the methodological choices in virome studies. For researchers primarily interested in RNA viruses, the dsRNA approach is advised because it consistently provided a more comprehensive description of the analysed phytoviromes, but it understandably underrepresented DNA viruses and bacteriophages.
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.subjectmetagenomics
dc.subjectOTU
dc.subject.endouble-stranded RNA (dsRNA)
dc.subject.envirion-associated nucleic acids (VANA)
dc.subject.envirome
dc.subject.enviral diversity
dc.subject.enphytovirome
dc.title.enPhytovirome Analysis of Wild Plant Populations: Comparison of Double-Stranded RNA and Virion-Associated Nucleic Acid Metagenomic Approaches.
dc.typeArticle de revue
dc.identifier.doi10.1128/JVI.01462-19
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalJournal of Virology
bordeaux.pagee01462-9
bordeaux.volume94
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02628250
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02628250v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Virology&rft.date=2020&rft.volume=94&rft.issue=1&rft.spage=e01462-9&rft.epage=e01462-9&rft.eissn=0022-538X&rft.issn=0022-538X&rft.au=MA,%20Yuxin&MARAIS,%20Armelle&LEFEBVRE,%20Marie&THEIL,%20S%C3%A9bastien&SVANELLA-DUMAS,%20Laurence&rft.genre=article


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record