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Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance.

hal.structure.identifierInternational Livestock Research Institute [CGIAR, Nairobi] [ILRI]
dc.contributor.authorSCHIECK, Elise
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE, Carole
hal.structure.identifierUniversität Bern = University of Bern = Université de Berne [UNIBE]
dc.contributor.authorFREY, Joachim
hal.structure.identifierDepartment of Biological Sciences
dc.contributor.authorVOZZA, Nicolas
hal.structure.identifierInstitute of Functional and Applied Anatomy
dc.contributor.authorHEGERMANN, Jan
hal.structure.identifierDepartment of Food Science
dc.contributor.authorMILLER, Rachel A
hal.structure.identifierWashington University School of Medicine [Saint Louis, MO]
dc.contributor.authorVALGUARNERA, Ezequiel
hal.structure.identifierInternational Livestock Research Institute [CGIAR, Nairobi] [ILRI]
dc.contributor.authorMURIUKI, Cecilia
hal.structure.identifierUniversity of Veterinary Medicine [Hannover]
dc.contributor.authorMEENS, Jochen
hal.structure.identifierInternational Livestock Research Institute [CGIAR, Nairobi] [ILRI]
dc.contributor.authorNENE, Vish
hal.structure.identifierInternational Livestock Research Institute [CGIAR, Nairobi] [ILRI]
dc.contributor.authorNAESSENS, Jan
hal.structure.identifierUniversité de Lausanne = University of Lausanne [UNIL]
dc.contributor.authorWEBER, Johann
hal.structure.identifierUniversity of Alberta
dc.contributor.authorLOWARY, Todd L
hal.structure.identifierJ. Craig Venter Institute [La Jolla, USA] [JCVI]
dc.contributor.authorVASHEE, Sanjay
hal.structure.identifierDepartment of Biological Sciences
dc.contributor.authorFELDMAN, Mario F
hal.structure.identifierInternational Livestock Research Institute [CGIAR, Nairobi] [ILRI]
dc.contributor.authorJORES, Joerg
dc.date.issued2016
dc.identifier.issn0950-382X
dc.description.abstractEnMycoplasma mycoides subsp. capri (Mmc) and subsp. mycoides (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis and septicaemia. They express galactofuranose residues on their surface, but their role in pathogenesis has not yet been determined. The M. mycoides genomes contain up to several copies of the glf gene, which encodes an enzyme catalyzing the last step in the synthesis of galactofuranose. We generated a deletion of the glf gene in a strain of Mmc using genome transplantation and tandem repeat endonuclease coupled cleavage (TREC) with yeast as an intermediary host for the genome editing. As expected, the resulting YCp1.1-Δglf strain did not produce the galactofuranose containing glycans as shown by immunoblots and immuno-electronmicroscopy employing a galactofuranose specific monoclonal antibody. The mutant lacking galactofuranose exhibited a decreased growth rate and a significantly enhanced adhesion to small ruminant cells. The mutant was also "leaking" as revealed by a β-galactosidase-based assay employing a membrane impermeable substrate. These findings indicate that galactofuranose-containing polysaccharides conceal adhesins and are important for membrane integrity. Unexpectedly, the mutant strain showed increased serum resistance.
dc.language.isoen
dc.publisherWiley
dc.subjectgalactan
dc.subjectgalactofuranose
dc.subjectmembrane
dc.subjectsynthetic genomics
dc.subject.enMycoplasma mycoides
dc.subject.enUDP-galactopyranose mutase
dc.subject.enadhesion
dc.subject.encarbohydrate
dc.title.enGalactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance.
dc.typeArticle de revue
dc.identifier.doi10.1111/mmi.13213
dc.subject.halSciences du Vivant [q-bio]/Biologie animale/Médecine vétérinaire et santé animal
dc.subject.halSciences du Vivant [q-bio]/Biologie animale
bordeaux.journalMolecular Microbiology
bordeaux.page55-70
bordeaux.volume99
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02631324
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02631324v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Microbiology&rft.date=2016&rft.volume=99&rft.issue=1&rft.spage=55-70&rft.epage=55-70&rft.eissn=0950-382X&rft.issn=0950-382X&rft.au=SCHIECK,%20Elise&LARTIGUE,%20Carole&FREY,%20Joachim&VOZZA,%20Nicolas&HEGERMANN,%20Jan&rft.genre=article


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