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Characterization by deep sequencing of divergent Plum bark necrosis stem pitting-associated virus isolates and development of a broad spectrum PBNSPaV detection assay.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFAURE, Chantal
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCOUTURE, Carole
hal.structure.identifierCentre de Lanxade
dc.contributor.authorBERGEY, Bernard
hal.structure.identifierCentre de Lanxade
dc.contributor.authorGENTIT, Pascal
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.issued2014
dc.identifier.issn0031-949X
dc.description.abstractEnPlum bark necrosis stem pitting-associated virus (PBNSPaV), the causal agent of plum bark necrosis stem pitting disease, belongs to the genus Ampelovirus in the family Closteroviridae. The complete genome sequence of PBNSPaV isolates from four Prunus sources was determined by pyrosequencing. All isolates showed the same genomic organization as the PBNSPaV reference isolate. The least divergent isolate, found in a peach tree from China, showed an overall 91.8% of nucleotide identity with the type isolate. Two closely related isolates, defining a second cluster of diversity, were found in two Japanese plums from France and showed only 82.8% identity with the type isolate. On the other hand, they were highly homologous with two recently described PBNSPaV divergent isolates from China. The fourth and most divergent isolate, from a Chinese peach, showed only 71.2% identity to other PBNSPaV isolates and was not detected by currently available PBNSPaV RT-PCR detection assays. Complete sequencing of the divergent isolates allowed the development of a more broad-spectrum detection test targeting a conserved region in the P61 gene. Taken together, these results indicate a much broader diversity of PBNSPaV than previously thought and provide for a more robust detection of this still poorly characterized pathogen.
dc.language.isoen
dc.publisherAmerican Phytopathological Society
dc.title.enCharacterization by deep sequencing of divergent Plum bark necrosis stem pitting-associated virus isolates and development of a broad spectrum PBNSPaV detection assay.
dc.typeArticle de revue
dc.identifier.doi10.1094/PHYTO-08-13-0229-R
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPhytopathology
bordeaux.page660-666
bordeaux.volume104
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-02631471
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02631471v1
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