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Integrative conjugative elements are widespread in field isolates of mycoplasma species pathogenic for ruminants

hal.structure.identifierUniversité de Lyon (COMUE)
hal.structure.identifierAgence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
dc.contributor.authorTARDY, Florence
hal.structure.identifierUniversité de Lyon (COMUE)
hal.structure.identifierAgence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
dc.contributor.authorMICK, Virginie
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
hal.structure.identifierUniversité de Toulouse [UT]
dc.contributor.authorDORDET FRISONI, Emilie
hal.structure.identifierUniversity of Melbourne
dc.contributor.authorMARENDA, Marc Serge
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBLANCHARD, Alain
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
hal.structure.identifierUniversité de Toulouse [UT]
dc.contributor.authorCITTI, Christine
dc.date.issued2015
dc.identifier.issn0099-2240
dc.description.abstractEnComparative genomics have revealed massive horizontal gene transfer (HGT) between Mycoplasma species sharing common ruminant hosts. Further results pointed toward an integrative conjugative element (ICE) as an important contributor of HGT in the small-ruminant-pathogen Mycoplasma agalactiae. To estimate the prevalence of ICEs in ruminant mycoplasmas, we surveyed their occurrence in a collection of 166 field strains representing 4 (sub) species that are recognized as major pathogens. Based on available sequenced genomes, we first defined the conserved, minimal ICE backbone as composed of 4 coding sequences (CDSs) that are evenly distributed and predicted to be essential for ICE chromosomal integration-excision and horizontal transfer. Screening of the strain collection revealed that these 4 CDSs are well represented in ruminant Mycoplasma species, suggesting widespread occurrence of ICEs. Yet their prevalence varies within and among species, with no correlation found with the individual strain history. Extrachromosomal ICE forms were also often detected, suggesting that ICEs are able to circularize in all species, a first and essential step in ICE horizontal transfer. Examination of the junction of the circular forms and comparative sequence analysis of conserved CDSs clearly pointed toward two types of ICE, the hominis and spiroplasma types, most likely differing in their mechanism of excision-integration. Overall, our data indicate the occurrence and maintenance of functional ICEs in a large number of field isolates of ruminant mycoplasmas. These may contribute to genome plasticity and gene exchanges and, presumably, to the emergence of diverse genotypes within pathogenic mycoplasmas of veterinary importance.
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.subjectevolution
dc.subjectstrain
dc.subjectmycoides cluster
dc.subjectagalactiae
dc.subject.enfamily
dc.subject.enswine
dc.subject.engenome sequence
dc.subject.endiversity
dc.subject.ensuppression-subtractive hybridization
dc.subject.enhyopneumoniae
dc.title.enIntegrative conjugative elements are widespread in field isolates of mycoplasma species pathogenic for ruminants
dc.typeArticle de revue
dc.identifier.doi10.1128/AEM.03723-14
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
dc.subject.halSciences du Vivant [q-bio]/Autre [q-bio.OT]
bordeaux.journalApplied and Environmental Microbiology
bordeaux.page1634-1643
bordeaux.volume81
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-02632194
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02632194v1
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