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Correlation-Based Network Analysis of Metabolite and Enzyme Profiles Reveals a Role of Citrate Biosynthesis in Modulating N and C Metabolism in Zea mays.

hal.structure.identifierBen-Gurion University of the Negev [BGU]
dc.contributor.authorTOUBIANA, David
hal.structure.identifierBen-Gurion University of the Negev [BGU]
dc.contributor.authorXUE, Wentao
hal.structure.identifierCornell University [New York]
dc.contributor.authorZHANG, Nengyi
hal.structure.identifierCornell University [New York]
dc.contributor.authorKREMLING, Karl
hal.structure.identifierCornell University [New York]
dc.contributor.authorGUR, Amit
hal.structure.identifierBen-Gurion University of the Negev [BGU]
dc.contributor.authorPILOSOF, Shai
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorSTITT, Mark
hal.structure.identifierCornell University [New York]
dc.contributor.authorBUCKLER, Edward S
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorFERNIE, Alisdair R
hal.structure.identifierBen-Gurion University of the Negev [BGU]
dc.contributor.authorFAIT, Aaron
dc.date.issued2016
dc.identifier.issn1664-462X
dc.description.abstractEnTo investigate the natural variability of leaf metabolism and enzymatic activity in a maize inbred population, statistical and network analyses were employed on metabolite and enzyme profiles. The test of coefficient of variation showed that sugars and amino acids displayed opposite trends in their variance within the population, consistently with their related enzymes. The overall higher CV values for metabolites as compared to the tested enzymes are indicative for their greater phenotypic plasticity. H(2) tests revealed galactinol (1) and asparagine (0.91) as the highest scorers among metabolites and nitrate reductase (0.73), NAD-glutamate dehydrogenase (0.52), and phosphoglucomutase (0.51) among enzymes. The overall low H(2) scores for metabolites and enzymes are suggestive for a great environmental impact or gene-environment interaction. Correlation-based network generation followed by community detection analysis, partitioned the network into three main communities and one dyad, (i) reflecting the different levels of phenotypic plasticity of the two molecular classes as observed for the CV values and (ii) highlighting the concerted changes between classes of chemically related metabolites. Community 1 is composed mainly of enzymes and specialized metabolites, community 2' is enriched in N-containing compounds and phosphorylated-intermediates. The third community contains mainly organic acids and sugars. Cross-community linkages are supported by aspartate, by the photorespiration amino acids glycine and serine, by the metabolically related GABA and putrescine, and by citrate. The latter displayed the strongest node-betweenness value (185.25) of all nodes highlighting its fundamental structural role in the connectivity of the network by linking between different communities and to the also strongly connected enzyme aldolase.
dc.language.isoen
dc.publisherFrontiers
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enTCA cycle
dc.subject.enZea mays
dc.subject.encorrelation-based network analysis
dc.subject.enenzymatic processes
dc.subject.enmetabolic networks and pathways
dc.subject.enmetabolism
dc.title.enCorrelation-Based Network Analysis of Metabolite and Enzyme Profiles Reveals a Role of Citrate Biosynthesis in Modulating N and C Metabolism in Zea mays.
dc.typeArticle de revue
dc.identifier.doi10.3389/fpls.2016.01022
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalFrontiers in Plant Science
bordeaux.page1022
bordeaux.volume7
bordeaux.peerReviewedoui
hal.identifierhal-02632485
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02632485v1
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