Adaptation of Lettuce mosaic virus to <em>Catharanthus roseus</em> involves mutations in the central domain of the VPg.
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | SVANELLA-DUMAS, Laurence | |
hal.structure.identifier | Unité de Pathologie Végétale [PV] | |
dc.contributor.author | VERDIN, Eric | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | FAURE, Chantal | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | GERMAN-RETANA, Sylvie | |
hal.structure.identifier | Unité de Pathologie Végétale [PV] | |
dc.contributor.author | GOGNALONS, Patrick | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | DANET, Jean-Luc | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | MARAIS, Armelle | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | CANDRESSE, Thierry | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0894-0282 | |
dc.description.abstractEn | An isolate of Lettuce mosaic virus (LMV, a Potyvirus) infecting Madagascar periwinckle (Catharanthus roseus) was identified and characterized by Illumina deep sequencing. LMV-Cr has no close affinities to previously sequenced LMV isolates and represents a novel, divergent LMV clade. Inoculation experiments with other representative LMV isolates showed that they are unable to infect C. roseus, which was not known to be a host for LMV. However, three C. roseus variants of one of these isolates, LMV-AF199, could be selected and partially or completely sequenced. These variants are characterized by the accumulation of mutations affecting the C-terminal part of the CI helicase and the central part of the VPg. In particular, a Serine to Proline mutation at amino acid 143 of the VPg was observed in all three independently selected variants and is also present in the LMV-Cr isolate, making it a prime candidate as a host range determinant. Other mutations at VPg positions 65 and 144 could also contribute to the ability to infect C. roseus. Inoculation experiments involving a recombinant LMV expressing a permissive lettuce translation initiation factor 4E (eIF4E) suggest that eIF4E does not contribute to the interaction of most LMV isolates with C. roseus. | |
dc.language.iso | en | |
dc.publisher | American Phytopathological Society | |
dc.rights.uri | http://creativecommons.org/licenses/by-sa/ | |
dc.subject | evolution | |
dc.subject.en | Potyvirus | |
dc.subject.en | VPg | |
dc.subject.en | host change | |
dc.subject.en | genome | |
dc.title.en | Adaptation of Lettuce mosaic virus to <em>Catharanthus roseus</em> involves mutations in the central domain of the VPg. | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1094/MPMI-10-13-0320-R | |
dc.subject.hal | Sciences du Vivant [q-bio]/Sciences agricoles | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie | |
bordeaux.journal | Molecular Plant-Microbe Interactions | |
bordeaux.page | 491-497 | |
bordeaux.volume | 27 | |
bordeaux.issue | 5 | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02632816 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Internationale | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02632816v1 | |
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