Evaluation of the genetic diversity of <em>Plum pox virus</em> in a single plum tree.
| hal.structure.identifier | Slovak Academy of Sciences [SAS] | |
| dc.contributor.author | PREDAJŇA, Lukáš | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | CANDRESSE, Thierry | |
| hal.structure.identifier | Slovak Academy of Sciences [SAS] | |
| dc.contributor.author | GLASA, Miroslav | |
| dc.date.issued | 2012 | |
| dc.identifier.issn | 0168-1702 | |
| dc.description.abstractEn | Genetic diversity of Plum pox virus (PPV) and its distribution within a single perennial woody host (plum, Prunus domestica) has been evaluated. A plum tree was triply infected by chip-budding with PPV-M, PPV-D and PPV-Rec isolates in 2003 and left to develop untreated under open field conditions. In September 2010 leaf and fruit samples were collected from different parts of the tree canopy. A 745-bp NIb-CP fragment of PPV genome, containing the hypervariable region encoding the CP N-terminal end was amplified by RT-PCR from each sample and directly sequenced to determine the dominant sequence. In parallel, the PCR products were cloned and a total of 105 individual clones were sequenced. Sequence analysis revealed that after 7 years of infection, only PPV-M was still detectable in the tree and that the two other isolates (PPV-Rec and PPV-D) had been displaced. Despite the fact that the analysis targeted a relatively short portion of the genome, a substantial amount of intra-isolate variability was observed for PPV-M. A total of 51 different haplotypes could be identified from the 105 individual sequences, two of which were largely dominant. However, no clear-cut structuration of the viral population by the tree architecture could be highlighted although the results obtained suggest the possibility of intra-leaf/fruit differentiation of the viral population. Comparison of the consensus sequence with the original source isolate showed no difference, suggesting within-plant stability of this original isolate under open field conditions. | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.subject | PPV | |
| dc.subject.en | Haplotype | |
| dc.subject.en | Prunus host | |
| dc.subject.en | Variability | |
| dc.title.en | Evaluation of the genetic diversity of <em>Plum pox virus</em> in a single plum tree. | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1016/j.virusres.2012.04.002 | |
| dc.subject.hal | Sciences du Vivant [q-bio] | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | |
| bordeaux.journal | Virus Research | |
| bordeaux.page | 112-7 | |
| bordeaux.volume | 167 | |
| bordeaux.issue | 1 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-02644209 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02644209v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Virus%20Research&rft.date=2012&rft.volume=167&rft.issue=1&rft.spage=112-7&rft.epage=112-7&rft.eissn=0168-1702&rft.issn=0168-1702&rft.au=PREDAJ%C5%87A,%20Luk%C3%A1%C5%A1&CANDRESSE,%20Thierry&GLASA,%20Miroslav&rft.genre=article |
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