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Silencing of the GDP-d-mannose 3,5-Epimerase Affects the Structure and Cross-linking of the Pectic Polysaccharide Rhamnogalacturonan II and Plant Growth in Tomato

hal.structure.identifierLaboratoire de Glycobiologie et Matrice Extracellulaire Végétale [Glyco-MEV]
dc.contributor.authorVOXEUR, Aline
hal.structure.identifierBiologie du Fruit
dc.contributor.authorGILBERT, Louise
hal.structure.identifierPolymères Biopolymères Surfaces [PBS]
dc.contributor.authorRIHOUEY, Christophe
hal.structure.identifierLaboratoire de Glycobiologie et Matrice Extracellulaire Végétale [Glyco-MEV]
dc.contributor.authorDRIOUICH, Azeddine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
hal.structure.identifierLaboratoire de Glycobiologie et Matrice Extracellulaire Végétale [Glyco-MEV]
dc.contributor.authorLEROUGE, Patrice
dc.date.issued2011-03-04
dc.identifier.issn0021-9258
dc.description.abstractEnL-galactose (L-Gal), a monosaccharide involved in L-ascorbate and rhamnogalacturonan II (RG-II) biosynthesis in plants, is produced in the cytosol by a GDP-D-mannose 3,5-epimerase (GME). It has been recently reported that the partial inactivation of GME induced growth defects affecting both cell division and cell expansion (Gilbert, L., Alhagdow, M., Nunes-Nesi, A., Quemener, B., Guillon, F., Bouchet, B., Faurobert, M., Gouble, B., Page, D., Garcia, V., Petit, J., Stevens, R., Causse, M., Fernie, A. R., Lahaye, M., Rothan, C., and Baldet, P. (2009) Plant J. 60, 499-508). In the present study, we show that the silencing of the two GME genes in tomato leaves resulted in approximately a 60% decrease in terminal L-Gal content in the side chain A of RG-II as well as in a lower capacity of RG-II to perform in muro cross-linking. In addition, we show that unlike supplementation with L-Gal or ascorbate, supplementation of GME-silenced lines with boric acid was able to restore both the wild-type growth phenotype of tomato seedlings and an efficient in muro boron-mediated cross-linking of RG-II. Our findings suggest that developmental phenotypes in GME-deficient lines are due to the structural alteration of RG-II and further underline the crucial role of the cross-linking of RG-II in the formation of the pectic network required for normal plant growth and development
dc.language.isoen
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dc.rights.urihttp://hal.archives-ouvertes.fr/licences/copyright/
dc.subject.en5-Epimerase
dc.subject.enl-Galactose
dc.subject.enRhamnogalacturonan II
dc.subject.enGDP-d-mannose 3
dc.subject.enCell Wall Galactose
dc.subject.enCarbohydrate Structure
dc.subject.enCarbohydrate Function
dc.subject.enCarbohydrate Biosynthesis
dc.title.enSilencing of the GDP-d-mannose 3,5-Epimerase Affects the Structure and Cross-linking of the Pectic Polysaccharide Rhamnogalacturonan II and Plant Growth in Tomato
dc.typeArticle de revue
dc.identifier.doi10.1074/jbc.M110.198614
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalJournal of Biological Chemistry
bordeaux.page8014 - 8020
bordeaux.volume286
bordeaux.issue10
bordeaux.peerReviewedoui
hal.identifierhal-01848364
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01848364v1
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