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hal.structure.identifierAzerbaijan National Academy of Sciences [ANAS]
dc.contributor.authorBALAKISHIYEVA, Gulnara
hal.structure.identifierInstitute of Horticulture and subtropical crops
dc.contributor.authorQURBANOV, Madat
hal.structure.identifierAzerbaijan National Academy of Sciences [ANAS]
dc.contributor.authorMAMMADOV, Alamdar
hal.structure.identifierAzerbaijan National Academy of Sciences [ANAS]
dc.contributor.authorBAYRAMOV, Shaniyar
hal.structure.identifierAzerbaijan National Academy of Sciences [ANAS]
dc.contributor.authorALIYEV, Jalal
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFOISSAC, Xavier
dc.date.issued2011
dc.identifier.issn0929-1873
dc.description.abstractEn‘Candidatus Phytoplasma brasiliense’, a phytoplasma taxon associated with hibiscus witches’ broom disease was first described in 2001 in Brazil. In September 2007, a peach tree (Prunus persica) displaying yellowing symptoms reminiscent of phytoplasma infection was sampled in Guba region of Azerbaijan. A phytoplasma was detected in the diseased peach tree by nested PCR amplification of its 16S rDNA with universal primers for phytoplasmas. Phylogenetical analyses of the amplified 16S rDNA showed that the phytoplasma infecting the peach tree corresponded to ‘Ca. P. brasiliense’, a species never reported in Euro-Mediterranean area. To set up a detection assay, cloning of a ‘Ca. P. brasiliense’ DNA fragment was undertaken by comparative RAPD. The amplified dnaK-dnaJ genetic locus was used to design a nested PCR assay able to amplify all ‘Ca. P. brasiliense’ isolates of the subgroup 16SrXV-A without amplifying the related members of the group 16SrII. This assay also allowed confirming the first detection of ‘Ca. P. brasiliense’ in diseased basil collected in south Lebanon.
dc.language.isoen
dc.publisherSpringer Verlag
dc.subjectPECHER
dc.subjectBACTERIE DU PHLOEME
dc.subject.enPHLOEM-RESTRICTED-BACTERIA
dc.subject.enNESTED-PCR
dc.subject.enRAPD
dc.subject.enCANDIDATUS PHYTOPLASMA BRASILIENSE
dc.title.enDetection of ‘Candidatus Phytoplasma brasiliense’ in a new geographic region and existence of two genetically distinct populations’
dc.typeArticle de revue
dc.identifier.doi10.1007/s10658-011-9773-7
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalEuropean Journal of Plant Pathology
bordeaux.page457-462
bordeaux.volume130
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-02646870
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02646870v1
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