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hal.structure.identifierAristotle University of Thessaloniki
dc.contributor.authorMATHIOUDAKIS, Matthaios M.
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierUniversity of Naples Federico II = Università degli studi di Napoli Federico II
dc.contributor.authorBARONE, Maria
hal.structure.identifierUniversity of Naples Federico II = Università degli studi di Napoli Federico II
dc.contributor.authorRAGOZZINO, Antonio
hal.structure.identifierAristotle University of Thessaloniki
dc.contributor.authorKATIS, Nikolaos I.
dc.date.issued2012
dc.identifier.issn0920-8569
dc.description.abstractEnJapanese quince, ornamental and wild pear symptomless samples were infected with Apple stem pitting virus (ASPV). Identification of ASPV was achieved by different PCR assays that amplified either the RNA polymerase or coat protein gene regions. For further confirmation, 312 bp amplicons within the polymerase gene were sequenced and compared with previously published ASPV sequences and additional sequences of isolates from ancient Italian cultivars. Comparison of the partial sequences isolated from wild/ornamental hosts and from cultivated species revealed significant divergence levels. Among the wild/ornamental isolates, the PCT88 isolate from Pyrus calleryana was the most divergent, having an amino acid deletion and incorporating a unique stretch of amino acids not present in any other isolate. Further to this preliminary partial sequence data, statistical analysis demonstrated that the isolates from wild or ornamental hosts were not more closely related to each other than to isolates from cultivated hosts. These results represent the first report of natural ASPV infection in these novel ornamental and wild Rosaceae hosts.
dc.language.isoen
dc.publisherSpringer Verlag
dc.subjectBIOLOGIE MOLECULAIRE
dc.subjectVIROLOGIE
dc.subject.enAPPLE STEM PITTING VIRUS
dc.subject.enASPV
dc.subject.enBETAFLEXIVIRIDAE
dc.subject.enROSACEE
dc.subject.enCYDONIA JAPONICA
dc.subject.enPYRUS CALLERYANA
dc.subject.enP. AMYGDALIFORMIS
dc.subject.enRT-PCR
dc.title.enCydonia japonica, Pyrus calleryana and P. amygdaliformis: three new ornamental or wild hosts of Apple stem pitting virus
dc.typeArticle de revue
dc.identifier.doi10.1007/s11262-011-0682-9
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalVirus Genes
bordeaux.page319-322
bordeaux.volume44
bordeaux.issue2
bordeaux.peerReviewedoui
hal.identifierhal-02647396
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647396v1
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