Cydonia japonica, Pyrus calleryana and P. amygdaliformis: three new ornamental or wild hosts of Apple stem pitting virus
BARONE, Maria
University of Naples Federico II = Università degli studi di Napoli Federico II [UNINA]
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University of Naples Federico II = Università degli studi di Napoli Federico II [UNINA]
BARONE, Maria
University of Naples Federico II = Università degli studi di Napoli Federico II [UNINA]
University of Naples Federico II = Università degli studi di Napoli Federico II [UNINA]
RAGOZZINO, Antonio
University of Naples Federico II = Università degli studi di Napoli Federico II [UNINA]
< Reduce
University of Naples Federico II = Università degli studi di Napoli Federico II [UNINA]
Language
en
Article de revue
This item was published in
Virus Genes. 2012, vol. 44, n° 2, p. 319-322
Springer Verlag
English Abstract
Japanese quince, ornamental and wild pear symptomless samples were infected with Apple stem pitting virus (ASPV). Identification of ASPV was achieved by different PCR assays that amplified either the RNA polymerase or coat ...Read more >
Japanese quince, ornamental and wild pear symptomless samples were infected with Apple stem pitting virus (ASPV). Identification of ASPV was achieved by different PCR assays that amplified either the RNA polymerase or coat protein gene regions. For further confirmation, 312 bp amplicons within the polymerase gene were sequenced and compared with previously published ASPV sequences and additional sequences of isolates from ancient Italian cultivars. Comparison of the partial sequences isolated from wild/ornamental hosts and from cultivated species revealed significant divergence levels. Among the wild/ornamental isolates, the PCT88 isolate from Pyrus calleryana was the most divergent, having an amino acid deletion and incorporating a unique stretch of amino acids not present in any other isolate. Further to this preliminary partial sequence data, statistical analysis demonstrated that the isolates from wild or ornamental hosts were not more closely related to each other than to isolates from cultivated hosts. These results represent the first report of natural ASPV infection in these novel ornamental and wild Rosaceae hosts.Read less <
Keywords
VIROLOGIE
BIOLOGIE MOLECULAIRE
English Keywords
RT-PCR
P. AMYGDALIFORMIS
PYRUS CALLERYANA
CYDONIA JAPONICA
ROSACEE
BETAFLEXIVIRIDAE
ASPV
APPLE STEM PITTING VIRUS
Origin
Hal importedCollections