Afficher la notice abrégée

The tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning.

hal.structure.identifierWeizmann Institute of Science [Rehovot, Israël]
hal.structure.identifierShanghai Jiao Tong University [Shanghai]
dc.contributor.authorSHI, Jian Xin
hal.structure.identifierWeizmann Institute of Science [Rehovot, Israël]
dc.contributor.authorADATO, Avital
hal.structure.identifierAgricultural Research Organization
dc.contributor.authorALKAN, Noam
hal.structure.identifierCornell University [New York]
dc.contributor.authorHE, Yonghua
hal.structure.identifierWeizmann Institute of Science [Rehovot, Israël]
dc.contributor.authorLASHBROOKE, Justin
hal.structure.identifierCornell University [New York]
dc.contributor.authorMATAS, Antonio J
hal.structure.identifierWeizmann Institute of Science [Rehovot, Israël]
dc.contributor.authorMEIR, Sagit
hal.structure.identifierWeizmann Institute of Science [Rehovot, Israël]
dc.contributor.authorMALITSKY, Sergey
hal.structure.identifierCornell University [New York]
dc.contributor.authorISAACSON, Tal
hal.structure.identifierAgricultural Research Organization
dc.contributor.authorPRUSKY, Dov
hal.structure.identifierWeizmann Institute of Science [Rehovot, Israël]
dc.contributor.authorLESHKOWITZ, Dena
hal.structure.identifierRheinische Friedrich-Wilhelms-Universität Bonn
dc.contributor.authorSCHREIBER, Lukas
hal.structure.identifierUniversitat Politècnica de València = Universitad Politecnica de Valencia = Polytechnic University of Valencia [UPV]
dc.contributor.authorGRANELL, Antonio R
hal.structure.identifierCentre National de la Recherche Scientifique [CNRS]
dc.contributor.authorGRAUSEM, Bernard
hal.structure.identifierCentre National de la Recherche Scientifique [CNRS]
dc.contributor.authorPINOT, Franck
hal.structure.identifierCornell University [New York]
dc.contributor.authorROSE, Jocelyn K C
hal.structure.identifierWeizmann Institute of Science [Rehovot, Israël]
dc.contributor.authorROGACHEV, Ilana
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierWeizmann Institute of Science [Rehovot, Israël]
dc.contributor.authorAHARONI, Asaph
dc.date.issued2013
dc.identifier.issn0028-646X
dc.description.abstractEnFleshy tomato fruit typically lacks stomata; therefore, a proper cuticle is particularly vital for fruit development and interaction with the surroundings. Here, we characterized the tomato SlSHINE3 (SlSHN3) transcription factor to extend our limited knowledge regarding the regulation of cuticle formation in fleshy fruits. We created SlSHN3 overexpressing and silenced plants, and used them for detailed analysis of cuticular lipid compositions, phenotypic characterization, and the study on the mode of SlSHN3 action. Heterologous expression of SlSHN3 in Arabidopsis phenocopied overexpression of the Arabidopsis SHNs. Silencing of SlSHN3 results in profound morphological alterations of the fruit epidermis and significant reduction in cuticular lipids. We demonstrated that SlSHN3 activity is mediated by control of genes associated with cutin metabolism and epidermal cell patterning. As with SlSHN3 RNAi lines, mutation in the SlSHN3 target gene, SlCYP86A69, resulted in severe cutin deficiency and altered fruit surface architecture. In vitro activity assays demonstrated that SlCYP86A69 possesses NADPH-dependent ω-hydroxylation activity, particularly of C18:1 fatty acid to the 18-hydroxyoleic acid cutin monomer. This study provided insights into transcriptional mechanisms mediating fleshy fruit cuticle formation and highlighted the link between cutin metabolism and the process of fruit epidermal cell patterning.
dc.language.isoen
dc.publisherWiley
dc.subjecttranscriptional regulation
dc.subject.encutin
dc.subject.enCYP86A69
dc.subject.enepidermal cell patterning
dc.subject.enSolanum lycopersicum
dc.subject.ensurface architecture
dc.subject.enwax
dc.title.enThe tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning.
dc.typeArticle de revue
dc.identifier.doi10.1111/nph.12032
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalNew Phytologist
bordeaux.page468-80
bordeaux.volume197
bordeaux.issue2
bordeaux.peerReviewedoui
hal.identifierhal-02647634
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647634v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=New%20Phytologist&rft.date=2013&rft.volume=197&rft.issue=2&rft.spage=468-80&rft.epage=468-80&rft.eissn=0028-646X&rft.issn=0028-646X&rft.au=SHI,%20Jian%20Xin&ADATO,%20Avital&ALKAN,%20Noam&HE,%20Yonghua&LASHBROOKE,%20Justin&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée