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Metabolic interaction between anthocyanin and lignin biosynthesis is associated with peroxidase FaPRX27 in strawberry fruit

hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorRING, Ludwig
hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorYEH, Su-Ying
hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorHÜCHERIG, Stephanie
hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorHOFFMANN, Thomas
hal.structure.identifierUniversidad de Córdoba = University of Córdoba [Córdoba]
dc.contributor.authorBLANCO-PORTALES, Rosario
hal.structure.identifierUnité de recherches Espèces Fruitières et Vigne [UREFV]
dc.contributor.authorFOUCHÉ, Mathieu
hal.structure.identifierUniversitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona [UAB]
dc.contributor.authorVILLATORO, Carmen
hal.structure.identifierUnité de recherches Espèces Fruitières et Vigne [UREFV]
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDENOYES, Beatrice
hal.structure.identifierUniversitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona [UAB]
dc.contributor.authorMONFORT, Amparo
hal.structure.identifierUniversidad de Córdoba = University of Córdoba [Córdoba]
dc.contributor.authorCABALLERO, José-Luis
hal.structure.identifierUniversidad de Córdoba = University of Córdoba [Córdoba]
dc.contributor.authorMUÑOZ-BLANCO, Juan
hal.structure.identifierMax Planck Institute for Chemical Ecology
dc.contributor.authorGERSHENSON, Jonathan
hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorSCHWAB, Wilfried
dc.date.issued2013
dc.identifier.issn0032-0889
dc.description.abstractEnPlant phenolics have drawn increasing attention due to their potential nutritional benefits. Although the basic reactions of the phenolics biosynthetic pathways in plants have been intensively analyzed, the regulation of their accumulation and flux through the pathway is not that well established. The aim of this study was to use a strawberry (Fragaria × ananassa) microarray to investigate gene expression patterns associated with the accumulation of phenylpropanoids, flavonoids, and anthocyanins in strawberry fruit. An examination of the transcriptome, coupled with metabolite profiling data from different commercial varieties, was undertaken to identify genes whose expression correlated with altered phenolics composition. Seventeen comparative microarray analyses revealed 15 genes that were differentially (more than 200-fold) expressed in phenolics-rich versus phenolics-poor varieties. The results were validated by heterologous expression of the peroxidase FaPRX27 gene, which showed the highest altered expression level (more than 900-fold). The encoded protein was functionally characterized and is assumed to be involved in lignin formation during strawberry fruit ripening. Quantitative trait locus analysis indicated that the genomic region of FaPRX27 is associated with the fruit color trait. Down-regulation of the CHALCONE SYNTHASE gene and concomitant induction of FaPRX27 expression diverted the flux from anthocyanins to lignin. The results highlight the competition of the different phenolics pathways for their common precursors. The list of the 15 candidates provides new genes that are likely to impact polyphenol accumulation in strawberry fruit and could be used to develop molecular markers to select phenolics-rich germplasm.
dc.language.isoen
dc.publisherOxford University Press ; American Society of Plant Biologists
dc.subject.enfragaria x ananassa
dc.subject.encultivated strawberry
dc.subject.enantioxidant capacity
dc.title.enMetabolic interaction between anthocyanin and lignin biosynthesis is associated with peroxidase FaPRX27 in strawberry fruit
dc.typeArticle de revue
dc.identifier.doi10.1104/pp.113.222778
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant Physiology
bordeaux.page43-60
bordeaux.volume163
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02647823
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647823v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Physiology&rft.date=2013&rft.volume=163&rft.issue=1&rft.spage=43-60&rft.epage=43-60&rft.eissn=0032-0889&rft.issn=0032-0889&rft.au=RING,%20Ludwig&YEH,%20Su-Ying&H%C3%9CCHERIG,%20Stephanie&HOFFMANN,%20Thomas&BLANCO-PORTALES,%20Rosario&rft.genre=article


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