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The C terminus of lettuce mosaic potyvirus cylindrical inclusion helicase interacts with the viral VPg and with lettuce translation eukaryotic initiation factor 4E

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorTAVERT-ROUDET, Genevieve
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorABDUL RAZZAK, Anas
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDOUBLET, Bénédicte
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorWALTER, Jocelyne
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDELAUNAY, Thierry
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMICHON, Thierry
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLE GALL, Olivier
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.issued2012
dc.identifier.issn0022-1317
dc.description.abstractEnRecessive resistance to lettuce mosaic virus (LMV) is conferred in lettuce by the mo1 gene, encoding the eukaryotic translation initiation factor 4E (eIF4E). The C terminus of the viral cylindrical inclusion helicase (CI-Cter), together with the VPg, is involved directly in overcoming mo1 resistance. In this study, recombinant LMV VPg and CI-Cter proteins from wild-type or resistance-breaking isolates were expressed and purified from Escherichia coli. The allelic forms of eIF4E from susceptible or resistant lettuce cultivars were produced similarly and these proteins were used in ELISA-based assays to demonstrate the in vitro binding of the various forms of LMV CI-Cter to both lettuce eIF4E and LMV VPg proteins. All combinations tested displayed significant and specific interactions, and the interaction between the C-terminal part of the LMV CI and eIF4E was confirmed in vivo in bimolecular fluorescence complementation assays. Higher interaction signals for both CI–eIF4E and CI–VPg were observed for LMV-E, indicating that the eIF4E interaction network involving CI and VPg appears to be stronger in the case of this resistance-breaking isolate. This could suggest the need for a minimal interaction threshold for infection success in resistant lettuce, but more precise measurement of the interaction parameters linking eIF4E, VPg and CI is needed in order to reinforce such a hypothesis.
dc.language.isoen
dc.publisherMicrobiology Society
dc.rights.urihttp://hal.archives-ouvertes.fr/licences/copyright/
dc.subjectVIROLOGIE
dc.subjectBIOLOGIE MOLECULAIRE
dc.title.enThe C terminus of lettuce mosaic potyvirus cylindrical inclusion helicase interacts with the viral VPg and with lettuce translation eukaryotic initiation factor 4E
dc.typeArticle de revue
dc.identifier.doi10.1099/vir.0.035881-0
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.journalJournal of General Virology
bordeaux.page184-193
bordeaux.volume93
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02652350
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02652350v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20General%20Virology&rft.date=2012&rft.volume=93&rft.issue=1&rft.spage=184-193&rft.epage=184-193&rft.eissn=0022-1317&rft.issn=0022-1317&rft.au=TAVERT-ROUDET,%20Genevieve&ABDUL%20RAZZAK,%20Anas&DOUBLET,%20B%C3%A9n%C3%A9dicte&WALTER,%20Jocelyne&DELAUNAY,%20Thierry&rft.genre=article


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