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Up-regulation of leucine aminopeptidase-A in cadmium-treated tomato roots

hal.structure.identifierUniversité de Tunis
dc.contributor.authorBOULILA-ZOGHLAMI, Latifa
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGALLUSCI, Philippe
hal.structure.identifierUniversity of California [Riverside] [UC Riverside]
dc.contributor.authorHOLZER, Frances M.
hal.structure.identifierUniversity of Nebraska System
dc.contributor.authorBASSET, Gilles J.
hal.structure.identifierUniversité de Tunis
dc.contributor.authorDJEBALI, Whabi
hal.structure.identifierUniversité de Tunis
dc.contributor.authorCHAIBI, Wided
hal.structure.identifierUniversity of Nebraska System
dc.contributor.authorWALLING, Linda L.
hal.structure.identifierInteractions Biotiques et Santé Végétale [IBSV]
dc.contributor.authorBROUQUISSE, Renaud
dc.date.issued2011
dc.identifier.issn0032-0935
dc.description.abstractEnThe effects of cadmium (Cd) on aminopeptidase (AP) activities and Leucine-AP (LAP) expression were investigated in the roots of tomato (Solanum lycopersicum L., var Ibiza) plants. Three-week-old plants were grown for 10 days in the presence of 0.3-300 μM Cd and compared to control plants grown in the absence of Cd. AP activities were measured using six different p-nitroanilide (p-NA) substrates. Leu, Met, Arg, Pro and Lys hydrolyzing activities increased in roots of Cd-treated plants, while Phe-pNA cleavage was not enhanced after Cd treatments. The use of peptidase inhibitors showed that most of the Leu-pNA hydrolyzing activity was related to one or several metallo-APs. Changes in Lap transcripts, protein and activities were measured in the roots of 0 and 30-μM Cd-treated plants. LapA transcript levels increased in Cd-treated roots, whereas LapN RNAs levels were not modified. To assess amount of Leu-pNA hydrolyzing activity associated with the hexameric LAPs, LAP activity was measured following immunoprecipitation with a LAP polyclonal antiserum. LAP activity increased in Cd-treated roots. There was a corresponding increase in LAP-A protein levels detected in 2D-immunoblots. The role of LAP-A in the proteolytic response to Cd stress is discussed.
dc.language.isoen
dc.publisherSpringer Verlag
dc.subjectBIOLOGIE VEGETALE
dc.subject.enLAP-A
dc.title.enUp-regulation of leucine aminopeptidase-A in cadmium-treated tomato roots
dc.typeArticle de revue
dc.identifier.doi10.1007/s00425-011-1468-y
dc.subject.halSciences du Vivant [q-bio]/Génétique/Génétique des plantes
bordeaux.journalPlanta
bordeaux.page857-863
bordeaux.volume234
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-02652540
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02652540v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Planta&rft.date=2011&rft.volume=234&rft.issue=4&rft.spage=857-863&rft.epage=857-863&rft.eissn=0032-0935&rft.issn=0032-0935&rft.au=BOULILA-ZOGHLAMI,%20Latifa&GALLUSCI,%20Philippe&HOLZER,%20Frances%20M.&BASSET,%20Gilles%20J.&DJEBALI,%20Whabi&rft.genre=article


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