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Adenine Nucleotide and Nicotinamide Adenine Dinucleotide Measurements in Plants

hal.structure.identifierMax-Planck-Institut für Molekulare Pflanzenphysiologie [MPI-MP]
hal.structure.identifierCenter of Plant Systems Biology and Biotechnology [Plovdiv] [CPSBB]
dc.contributor.authorZHANG, Youjun
hal.structure.identifierMax-Planck-Institut für Molekulare Pflanzenphysiologie [MPI-MP]
dc.contributor.authorKRAHNERT, Ina
hal.structure.identifierMax-Planck-Institut für Molekulare Pflanzenphysiologie [MPI-MP]
dc.contributor.authorBOLZE, Antje
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
hal.structure.identifierCenter of Plant Systems Biology and Biotechnology [Plovdiv] [CPSBB]
dc.contributor.authorFERNIE, Alisdair
dc.date.issued2020
dc.description.abstractEnAs the principal co-factors of many metabolic pathways, the measurement of both adenine nucleotides and nicotinamide adenine dinucleotide provides important information about cellular energy metabolism. However, given their rapid and reversible conversion as well as their relatively low concentration ranges, it is difficult to measure these compounds. Here, we describe a highly sensitive and selective ion-pairing HPLC method with fluorescence detection to quantify adenine nucleotides in plants. In addition, nicotinamide adenine dinucleotide is a crucially important redox-active substrate for multiple catabolic and anabolic reactions with the ratios of NAD + /NADH and NADP + /NADPH being suggested as indicators of the general intracellular re-dox potential and hence metabolic state. Here, we describe highly sensitive enzyme cycling−based colorimetric assays (with a detection limit in the pmol range) performed subsequent to a simple extraction procedure involving acid or base extraction to allow the measurement of the cellular levels of these metabo-lites. © 2020 The Authors. Basic Protocol 1: Preparation of plant material for the measurement Basic Protocol 2: Measurement of ATP, ADP, and AMP via HPLC Basic Protocol 3: NAD + /NADP + measurements Basic Protocol 4: NADH/NADPH measurements Basic Protocol 5: Data analysis and quality control approaches Keywords: ADP r ATP r NAD + /NADP + r NADH/NADPH
dc.description.sponsorshipCentre français de phénomique végétale - ANR-11-INBS-0012
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectMétabolisme
dc.subjectPlante
dc.subjectPhysiologie végétale
dc.subject.enADP
dc.subject.enATP
dc.subject.enNAD+/NADP+
dc.subject.enNADH/NADPH
dc.title.enAdenine Nucleotide and Nicotinamide Adenine Dinucleotide Measurements in Plants
dc.typeArticle de revue
dc.identifier.doi10.1002/cppb.20115
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Amélioration des plantes
bordeaux.journalCurrent Protocols in Plant Biology
bordeaux.pagee20115
bordeaux.volume5
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-02926938
hal.version1
hal.popularnon
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02926938v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Current%20Protocols%20in%20Plant%20Biology&rft.date=2020&rft.volume=5&rft.issue=3&rft.spage=e20115&rft.epage=e20115&rft.au=ZHANG,%20Youjun&KRAHNERT,%20Ina&BOLZE,%20Antje&GIBON,%20Yves&FERNIE,%20Alisdair&rft.genre=article


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