Adenine Nucleotide and Nicotinamide Adenine Dinucleotide Measurements in Plants
ZHANG, Youjun
Max-Planck-Institut für Molekulare Pflanzenphysiologie [MPI-MP]
Center of Plant Systems Biology and Biotechnology [Plovdiv] [CPSBB]
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Max-Planck-Institut für Molekulare Pflanzenphysiologie [MPI-MP]
Center of Plant Systems Biology and Biotechnology [Plovdiv] [CPSBB]
ZHANG, Youjun
Max-Planck-Institut für Molekulare Pflanzenphysiologie [MPI-MP]
Center of Plant Systems Biology and Biotechnology [Plovdiv] [CPSBB]
Max-Planck-Institut für Molekulare Pflanzenphysiologie [MPI-MP]
Center of Plant Systems Biology and Biotechnology [Plovdiv] [CPSBB]
FERNIE, Alisdair
Max Planck Institute of Molecular Plant Physiology [MPI-MP]
Center of Plant Systems Biology and Biotechnology [Plovdiv] [CPSBB]
< Reduce
Max Planck Institute of Molecular Plant Physiology [MPI-MP]
Center of Plant Systems Biology and Biotechnology [Plovdiv] [CPSBB]
Language
en
Article de revue
This item was published in
Current Protocols in Plant Biology. 2020, vol. 5, n° 3, p. e20115
English Abstract
As the principal co-factors of many metabolic pathways, the measurement of both adenine nucleotides and nicotinamide adenine dinucleotide provides important information about cellular energy metabolism. However, given their ...Read more >
As the principal co-factors of many metabolic pathways, the measurement of both adenine nucleotides and nicotinamide adenine dinucleotide provides important information about cellular energy metabolism. However, given their rapid and reversible conversion as well as their relatively low concentration ranges, it is difficult to measure these compounds. Here, we describe a highly sensitive and selective ion-pairing HPLC method with fluorescence detection to quantify adenine nucleotides in plants. In addition, nicotinamide adenine dinucleotide is a crucially important redox-active substrate for multiple catabolic and anabolic reactions with the ratios of NAD + /NADH and NADP + /NADPH being suggested as indicators of the general intracellular re-dox potential and hence metabolic state. Here, we describe highly sensitive enzyme cycling−based colorimetric assays (with a detection limit in the pmol range) performed subsequent to a simple extraction procedure involving acid or base extraction to allow the measurement of the cellular levels of these metabo-lites. © 2020 The Authors. Basic Protocol 1: Preparation of plant material for the measurement Basic Protocol 2: Measurement of ATP, ADP, and AMP via HPLC Basic Protocol 3: NAD + /NADP + measurements Basic Protocol 4: NADH/NADPH measurements Basic Protocol 5: Data analysis and quality control approaches Keywords: ADP r ATP r NAD + /NADP + r NADH/NADPHRead less <
Keywords
Métabolisme
Plante
Physiologie végétale
English Keywords
ADP
ATP
NAD+/NADP+
NADH/NADPH
ANR Project
Centre français de phénomique végétale - ANR-11-INBS-0012
Origin
Hal importedCollections