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Proton-NMR Metabolomics of Rainbow Trout Fed a Plant-Based Diet Supplemented with Graded Levels of a Protein-Rich Yeast Fraction Reveal Several Metabolic Processes Involved in Growth

hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierNutrition, Métabolisme, Aquaculture [NuMéA]
hal.structure.identifierPhileo Lesaffre Animal Care
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorROQUES, Simon
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorDEBORDE, Catherine
hal.structure.identifierPhileo Lesaffre Animal Care
dc.contributor.authorRICHARD, Nadege
hal.structure.identifierCoopérative Le Gouessant [Lamballe]
dc.contributor.authorMARCHAND, Yann
hal.structure.identifierNutrition, Métabolisme, Aquaculture [NuMéA]
dc.contributor.authorLARROQUET, Laurence
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPRIGENT, Sylvain
hal.structure.identifierNutrition, Métabolisme, Aquaculture [NuMéA]
dc.contributor.authorSKIBA-CASSY, Sandrine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorMOING, Annick
hal.structure.identifierNutrition, Métabolisme, Aquaculture [NuMéA]
dc.contributor.authorFAUCONNEAU, Benoit
dc.date.issued2020
dc.identifier.issn0022-3166
dc.description.abstractEnBackgroundPlant raw materials are commonly used in aquafeeds, as marine resources are unsustainable. However, full plant-based diets lead to poorer fish growth performance.ObjectiveWe aimed to understand the metabolic effects of a yeast fraction as a protein supplement in a plant-based diet and to integrate such effects with phenotypic traits as a new approach to assess the interest of this raw material.MethodsJuvenile (49 g) rainbow trout (Oncorhynchus mykiss) were fed graded levels of a yeast protein-rich fraction (5% YST05, 10% YST10, 15% YST15) in a plant-based diet (PB) for 84 d. Final body weight, feed conversion ratio, and hepatosomatic and viscerosomatic indexes were measured. Plasma, liver, and muscle 1H-NMR fingerprints were analyzed with principal component analyses, and their metabolite patterns were clustered according to the yeast level to identify concomitant metabolic effects. A regression modeling approach was used to predict tissue metabolite changes from plasma fingerprints.ResultsIn tissues, the patterns of metabolite changes followed either linear trends with the gradual inclusion of a yeast fraction (2 patterns out of 6 in muscle, 1 in liver) or quadratic trends (4 patterns in muscle, 5 in liver). Muscle aspartate and glucose (395 and 138% maximum increase in relative content compared with PB, respectively) revealing modification in energy metabolism, as well as modification of liver betaine (163% maximum increase) and muscle histidine (57% maximum decrease) related functions, indicates that the yeast fraction could improve growth in several ways. The highest correlation between measured and predicted metabolite intensities in a tissue based on plasma fingerprints was observed for betaine in liver (r = 0.80).ConclusionsThese findings herald a new approach to assess the plurality of metabolic effects induced by diets and establish the optimal level of raw materials. They open the way for using plasma as a noninvasive matrix in trout nutrition studies.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
dc.language.isoen
dc.publisherAmerican Society for Nutrition
dc.subject.enNMR
dc.subject.enfish nutrition
dc.subject.enmetabolic process
dc.subject.enmetabolomics
dc.subject.enrainbow trout
dc.subject.enyeast
dc.title.enProton-NMR Metabolomics of Rainbow Trout Fed a Plant-Based Diet Supplemented with Graded Levels of a Protein-Rich Yeast Fraction Reveal Several Metabolic Processes Involved in Growth
dc.typeArticle de revue
dc.identifier.doi10.1093/jn/nxaa206
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie animale
dc.subject.halSciences du Vivant [q-bio]/Alimentation et Nutrition
bordeaux.journalJournal of Nutrition
bordeaux.page2268-2277
bordeaux.volume150
bordeaux.issue9
bordeaux.peerReviewedoui
hal.identifierhal-02927022
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02927022v1
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