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Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus

hal.structure.identifierInstituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research [IVIA]
dc.contributor.authorRUIZ-GARCÍA, Ana Belén
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierInstituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research [IVIA]
dc.contributor.authorCANALES, Celia
hal.structure.identifierInstituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research [IVIA]
dc.contributor.authorMORÁN, Félix
hal.structure.identifierUniversidade Federal do Rio Grande do Sul [Porto Alegre] [UFRGS]
dc.contributor.authorMACHADO DE OLIVEIRA, Carlos
hal.structure.identifierUniversidade Federal do Rio Grande do Sul [Porto Alegre] [UFRGS]
dc.contributor.authorBERTOLINI, Edson
hal.structure.identifierInstituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research [IVIA]
dc.contributor.authorOLMOS, Antonio
dc.date.issued2020
dc.identifier.issn2223-7747
dc.description.abstractEnGenome organization and phylogenetic relationships of olive leaf yellowing-associated virus (OLYaV) with other members of the Closteroviridae family were determined. The complete coding sequence of OLYaV was obtained by high throughput sequencing of total RNA from a 35-year-old olive tree (cv. Zarzaleña) from Brazil, showing olive leaf yellowing disease and deformations in the wood. This represents the first report of OLYaV in this country. A genomic sequence of 16,700 nt containing 11 open reading frames (ORFs) was recovered, representing the complete virus coding capacity. The knowledge of the nucleotide sequence of the genome including the gene that codes the coat protein will facilitate the development of diagnostic tests, which are limited so far to PCR-based methods targeting the HSP70h gene. Interestingly, a thaumatin-like protein (ORF2), previously reported in other unassigned viruses in the Closteroviridae family, persimmon virus B and actidinia virus 1, was identified in the OLYaV genome. Phylogenetic analysis of shared proteins (ORF1a, ORF1b, HSP70h, HSP90h and CP) with all members of the Closteroviridae family provides new insight into the taxonomic position of these three closteroviruses and suggests they could represent a new genus in the family.
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectvirologie végétale
dc.subjectvirus phytopathogène
dc.subject.enClosteroviridae
dc.subject.enthaumatin-like protein
dc.subject.engenome
dc.subject.enhigh-throughput sequencing
dc.title.enMolecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus
dc.typeArticle de revue
dc.identifier.doi10.3390/plants9101272
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalPlants
bordeaux.page1272
bordeaux.volume9
bordeaux.issue10
bordeaux.peerReviewedoui
hal.identifierhal-02955256
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02955256v1
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