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The NAD Kinase Slr0400 Functions as a Growth Repressor in Synechocystis sp. PCC 6803

hal.structure.identifierHeinrich Heine Universität Düsseldorf = Heinrich Heine University [Düsseldorf]
hal.structure.identifierInstitute of Transformative Bio-Molecules [Nagoya, Japan]
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorISHIKAWA, Yuuma
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCASSAN, Cedric
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorKADEER, Aikeranmu
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorYUASA, Koki
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorSATO, Nozomu
hal.structure.identifierDepartment of Science and Engineering [University of Waseda]
dc.contributor.authorSONOIKE, Kintake 
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorKANEKO, Yasuko
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorMIYAGI, Atsuko
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorTAKAHASHI, Hiroko
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorISHIKAWA, Toshiki
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorYAMAGUCHI, Masatoshi
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorNISHIYAMA, Yoshitaka
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorHIHARA, Yukako
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierGraduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
dc.contributor.authorKAWAI-YAMADA, Maki
dc.date.issued2021
dc.identifier.issn0032-0781
dc.description.abstractEnNADP+, the phosphorylated form of nicotinamide adenine dinucleotide (NAD), plays an essential role in many cellular processes. NAD kinase (NADK), which is conserved in all living organisms, catalyzes the phosphorylation of NAD+ to NADP+. However, the physiological role of phosphorylation of NAD+ to NADP+ in the cyanobacterium Synechocystis remains unclear. In this study, we report that slr0400, an NADK-encoding gene in Synechocystis, functions as a growth repressor under light-activated heterotrophic growth conditions and light and dark cycle conditions in the presence of glucose. We show, via characterization of NAD(P)(H) content and enzyme activity, that NAD+ accumulation in slr0400-deficient mutant results in the unsuppressed activity of glycolysis and tricarboxylic acid (TCA) cycle enzymes. In determining whether Slr0400 functions as a typical NADK, we found that constitutive expression of slr0400 in an Arabidopsis nadk2-mutant background complements the pale-green phenotype. Moreover, to determine the physiological background behind the growth advantage of mutants lacking slr04000, we investigated the photobleaching phenotype of slr0400-deficient mutant under high-light conditions. Photosynthetic analysis found in the slr0400-deficient mutant resulted from malfunctions in the Photosystem II (PSII) photosynthetic machinery. Overall, our results suggest that NADP(H)/NAD(H) maintenance by slr0400 plays a significant role in modulating glycolysis and the TCA cycle to repress the growth rate and maintain the photosynthetic capacity.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subject.enCyanobacteria
dc.subject.enGrowth repressor
dc.subject.enNAD kinase
dc.subject.enSlr0400
dc.subject.enSynechocysti ssp. PCC 6803
dc.title.enThe NAD Kinase Slr0400 Functions as a Growth Repressor in Synechocystis sp. PCC 6803
dc.typeArticle de revue
dc.identifier.doi10.1093/pcp/pcab023
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant and Cell Physiology
bordeaux.page668-677
bordeaux.volume62
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-03182729
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03182729v1
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