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Validated MAGIC and GWAS population mapping reveals the link between vitamin E content and natural variation in chorismate metabolism in tomato

hal.structure.identifierInstituto de Fisiología, Biología Molecular y Neurociencias [Buenos Aires] [IFIBYNE]
dc.contributor.authorBURGOS, Estanislao
hal.structure.identifierInstituto de Fisiología, Biología Molecular y Neurociencias [Buenos Aires] [IFIBYNE]
dc.contributor.authorBELEN DE LUCA, Maria
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorDIOUF, Isidore
hal.structure.identifierInstituto de Fisiología, Biología Molecular y Neurociencias [Buenos Aires] [IFIBYNE]
dc.contributor.authorHARO, Luis
hal.structure.identifierMichigan State University System
dc.contributor.authorALBERT, Elise
hal.structure.identifierSyngenta France
dc.contributor.authorSAUVAGE, Christopher
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorTAO, Zhao
hal.structure.identifierInstituto Nacional de Tecnología Agropecuaria [INTA]
dc.contributor.authorBERMUDEZ, Luisa
hal.structure.identifierCentro de Investigaciones en Bioquímica Clínica e Inmunología [Córdoba] [CIBICI]
dc.contributor.authorASÍS, Ramon
hal.structure.identifierUniversidade Federal de Viçosa [Brasil] = Federal University of Viçosa [Brazil] = Université fédérale de Viçosa [Brésil] [UFV [Brésil]]
dc.contributor.authorNESI, Adriano
hal.structure.identifierPhysiologie cellulaire et végétale [LPCV]
dc.contributor.authorMATRINGE, Michel
hal.structure.identifierPhysiologie cellulaire et végétale [LPCV]
dc.contributor.authorBRÉHÉLIN, Claire
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGUIRAUD, Thomas
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERRAND, Carine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorATIENZA, Isabelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJORLY, Joana
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUXION, Jean-Philippe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
hal.structure.identifierMax-Planck-Institut
dc.contributor.authorFERNIE, Alisdair
hal.structure.identifierInstitut de biologie de l'ENS Paris [IBENS]
dc.contributor.authorQUADRANA, Leandro
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorCAUSSE, Mathilde
hal.structure.identifierInstituto de Fisiología, Biología Molecular y Neurociencias [Buenos Aires] [IFIBYNE]
dc.contributor.authorCARRARI, Fernando
dc.date.issued2021-02
dc.identifier.issn0960-7412
dc.description.abstractEnTocochromanols constitute the different forms of vitamin E (VTE), essential components of the human diet, and display a high membrane protectant activity. By combining interval mapping and genome-wide association studies (GWAS), we unveiled the genetic determinants of tocochromanol accumulation in tomato (Solanum lycopersicum) fruits. To enhance the nutritional value of this highly consumed vegetable, we dissected the natural intraspecific variability of tocochromanols in tomato fruits and genetically engineered their biosynthetic pathway. These analyses allowed the identification of a total of 25 quantitative trait loci interspersed across the genome pinpointing the chorismate-tyrosine pathway as a regulatory hub controlling the supply of the aromatic head group for tocochromanol biosynthesis. To validate the link between the chorismate-tyrosine pathway and VTE, we engineered tomato plants to bypass the pathway at the arogenate branch point. Transgenic tomatoes showed moderate increments in tocopherols (up to approximately 20%) and a massive accumulation of tocotrienols (up to approximately 3400%). Gene expression analyses of these plants reveal a trade-off between VTE and natural variation in chorismate metabolism explained by transcriptional reprogramming of specific structural genes of the pathway. By restoring the accumulation of alpha-tocotrienols (alpha-t3) in fruits, the plants produced here are of high pharmacological and nutritional interest.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation
dc.language.isoen
dc.publisherWiley
dc.subject.enSolanum lycopersicum
dc.subject.entomato fruit
dc.subject.enlvitamin E
dc.subject.entocotrieno
dc.subject.entocopherol
dc.title.enValidated MAGIC and GWAS population mapping reveals the link between vitamin E content and natural variation in chorismate metabolism in tomato
dc.typeArticle de revue
dc.identifier.doi10.1111/tpj.15077
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalThe Plant Journal
bordeaux.page907-923
bordeaux.volume105
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-03347595
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03347595v1
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