hal.structure.identifier | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]] | |
dc.contributor.author | LEBAS, Benedicte | |
hal.structure.identifier | Fera Science Ltd | |
dc.contributor.author | ADAMS, Ian | |
hal.structure.identifier | University of California [Davis] [UC Davis] | |
dc.contributor.author | AL RWAHNIH, Maher | |
hal.structure.identifier | Research Institute for Agricultural, Fisheries and Food [ILVO] | |
dc.contributor.author | BAEYEN, Steve | |
hal.structure.identifier | Canadian Food Inspection Agency [CFIA] | |
dc.contributor.author | BILODEAU, Guillaume J. | |
hal.structure.identifier | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]] | |
dc.contributor.author | BLOUIN, Arnaud | |
hal.structure.identifier | Newcastle University [Newcastle] | |
dc.contributor.author | BOONHAM, Neil | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | CANDRESSE, Thierry | |
hal.structure.identifier | Centre wallon de Recherches Agronomiques [Belgique] = Walloon Agricultural Research Centre [Belgium] [CRA-W] | |
dc.contributor.author | CHANDELIER, Anne | |
hal.structure.identifier | Research Institute for Agricultural, Fisheries and Food [ILVO] | |
dc.contributor.author | DE JONGHE, Kris | |
hal.structure.identifier | Fera Science Ltd | |
dc.contributor.author | FOX, Adrian | |
hal.structure.identifier | Institute for Epidemiology and Pathogen Diagnostics | |
dc.contributor.author | GAAFAR, Yahya | |
hal.structure.identifier | Laboratoire de santé des végétaux [LSV Angers] | |
dc.contributor.author | GENTIT, Pascal | |
hal.structure.identifier | Research Institute for Agricultural, Fisheries and Food [ILVO] | |
dc.contributor.author | HAEGEMAN, Annelies | |
hal.structure.identifier | Ministry for Primary Industries | |
dc.contributor.author | HO, Wellcome | |
hal.structure.identifier | Animal Plant Health Inspection Service, APHIS-USDA, Humble, Texas, 77338, USA. | |
dc.contributor.author | HURTADO-GONZALES, Oscar | |
dc.contributor.author | JONKERS, Wilfried | |
hal.structure.identifier | World Agroforestry Center [CGIAR, Pérou] [ICRAF] | |
dc.contributor.author | KREUZE, Jan | |
hal.structure.identifier | National Institute of Biology [Ljubljana] [NIB] | |
dc.contributor.author | KUTJNAK, Denis | |
hal.structure.identifier | Centro de Investigaciones Biológicas [CSIC] | |
dc.contributor.author | LANDA, Blanca | |
hal.structure.identifier | University of Tasmania [Hobart] [UTAS] | |
dc.contributor.author | LIU, Mingxin | |
hal.structure.identifier | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]] | |
dc.contributor.author | MACLOT, François | |
hal.structure.identifier | Animal Plant Health Inspection Service, APHIS-USDA, Humble, Texas, 77338, USA. | |
dc.contributor.author | MALAPI-WIGHT, Martha | |
hal.structure.identifier | Stellenbosch University | |
hal.structure.identifier | Citrus Research International | |
dc.contributor.author | MAREE, Hano | |
hal.structure.identifier | Agriculture Victoria [AgriBio] | |
dc.contributor.author | MARTONI, Francesco | |
hal.structure.identifier | National Institute of Biology [Ljubljana] [NIB] | |
dc.contributor.author | MEHLE, Natasha | |
hal.structure.identifier | CNR Istituto per la Protezione Sostenibile delle Piante [Torino, Italia] [IPSP] | |
dc.contributor.author | MINAFRA, Angelantonio | |
hal.structure.identifier | Horticultural Crops Research Unit | |
dc.contributor.author | MOLLOV, Dimitre | |
hal.structure.identifier | International Plant Protection Convention | |
dc.contributor.author | MOREIRA, Adriana | |
hal.structure.identifier | Animal Plant Health Inspection Service, APHIS-USDA, Humble, Texas, 77338, USA. | |
dc.contributor.author | NAKHLA, Mark | |
hal.structure.identifier | European and Mediterranean Plant Protection Organization - Organisation Européenne et Méditerranéenne pour la Protection des Plantes [EPPO] | |
dc.contributor.author | PETTER, Françoise | |
hal.structure.identifier | Agriculture Victoria [AgriBio] | |
dc.contributor.author | PIPER, Alexander | |
hal.structure.identifier | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]] | |
dc.contributor.author | PONCHART, Julien | |
hal.structure.identifier | Liverpool John Moores University [LJMU] | |
dc.contributor.author | RAE, Robbie | |
hal.structure.identifier | Laboratoire de santé des végétaux [LSV Angers] | |
dc.contributor.author | REMENANT, Benoit | |
hal.structure.identifier | Animal Plant Health Inspection Service, APHIS-USDA, Humble, Texas, 77338, USA. | |
dc.contributor.author | RIVERA, Yazmin | |
hal.structure.identifier | Agriculture Victoria [AgriBio] | |
dc.contributor.author | RODONI, Brendan | |
hal.structure.identifier | Netherlands Food and Consumer Product Safety Authority [NVWA] | |
dc.contributor.author | ROENHORST, Johanna W. | |
hal.structure.identifier | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]] | |
dc.contributor.author | ROLLIN, Johan | |
hal.structure.identifier | CNR Istituto per la Protezione Sostenibile delle Piante [Torino, Italia] [IPSP] | |
dc.contributor.author | SALDARELLI, Pasquale | |
hal.structure.identifier | Finnish Food Authority | |
dc.contributor.author | SANTALA, Johanna | |
hal.structure.identifier | International Seed Federation [ISF] | |
dc.contributor.author | SOUZA-RICHARDS, Rose | |
hal.structure.identifier | Università degli studi di Torino = University of Turin [UNITO] | |
dc.contributor.author | SPADARO, Davide | |
hal.structure.identifier | University of Exeter | |
dc.contributor.author | STUDHOLME, David | |
hal.structure.identifier | Canadian Food Inspection Agency [CFIA] | |
dc.contributor.author | SULTMANIS, Stefanie | |
hal.structure.identifier | Wageningen University and Research [Wageningen] [WUR] | |
dc.contributor.author | VAN DER VLUGT, René | |
hal.structure.identifier | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]] | |
dc.contributor.author | TAMISIER, Lucie | |
hal.structure.identifier | European and Mediterranean Plant Protection Organization - Organisation Européenne et Méditerranéenne pour la Protection des Plantes [EPPO] | |
dc.contributor.author | TRONTIN, Charlotte | |
hal.structure.identifier | Fera Science Ltd | |
dc.contributor.author | VAZQUEZ-IGLESIAS, Ines | |
hal.structure.identifier | Instituto Nacional de Investigação Agrária e Veterinária = National Institute for Agrarian and Veterinary Research [Oeiras, Portugal] [INIAV] | |
dc.contributor.author | VICENTE, Claudia | |
hal.structure.identifier | Netherlands Food and Consumer Product Safety Authority [NVWA] | |
dc.contributor.author | VOSSENBERG, Bart | |
hal.structure.identifier | Dienstleistungszentrum Ländlicher Raum DLR Rheinpfalz [DLR Rheinpfalz] | |
dc.contributor.author | WETZEL, Thierry | |
hal.structure.identifier | Julius Kühn-Institute | |
dc.contributor.author | ZIEBELL, Heiko | |
hal.structure.identifier | Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]] | |
dc.contributor.author | MASSART, Sebastien | |
dc.date.issued | 2022 | |
dc.identifier.issn | 0250-8052 | |
dc.description.abstractEn | High-throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in a sample. The growing interest in the application of HTS technologies for routine diagnostics in plant health laboratories is triggering the development of guidelines on how to prepare laboratories for performing HTS testing. This paper describes general and technical recommendations to guide laboratories through the complex process of preparing a laboratory for HTS tests within existing quality assurance systems. From nucleic acid extractions to data analysis and interpretation, all of the steps are covered to ensure reliable and reproducible results. These guidelines are relevant for the detection and identification of any plant pest (e.g. arthropods, bacteria, fungi, nematodes, invasive plants or weeds, protozoa, viroids, viruses), and from any type of matrix (e.g. pure microbial culture, plant tissue, soil, water), regardless of the HTS technology (e.g. amplicon sequencing, shotgun sequencing) and of the application (e.g. surveillance programme, phytosanitary certification, quarantine, import control). These guidelines are written in general terms to facilitate the adoption of HTS technologies in plant pest routine diagnostics and enable broader application in all plant health fields, including research. A glossary of relevant terms is provided among the Supplementary Material. | |
dc.language.iso | en | |
dc.publisher | Wiley | |
dc.subject.en | high-throughput sequencing | |
dc.subject.en | plant pest diagnostic test | |
dc.title.en | Facilitating the adoption of high‐throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step‐by‐step description | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1111/epp.12863 | |
dc.subject.hal | Sciences du Vivant [q-bio] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
dc.description.sponsorshipEurope | Validation of diagnostic tests to support plant health | |
bordeaux.journal | EPPO Bulletin | |
bordeaux.page | 394-418 | |
bordeaux.volume | 52 | |
bordeaux.issue | 2 | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-03753454 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Internationale | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-03753454v1 | |
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