Afficher la notice abrégée

Single NanoParticle Photothermal Tracking (SNaPT) of 5 nm gold beads in live cells

hal.structure.identifierCentre de physique moléculaire optique et hertzienne [CPMOH]
dc.contributor.authorLASNE, David
hal.structure.identifierCentre de physique moléculaire optique et hertzienne [CPMOH]
dc.contributor.authorA. BLAB, Gerhard
hal.structure.identifierCentre de physique moléculaire optique et hertzienne [CPMOH]
dc.contributor.authorBERCIAUD, Stéphane
hal.structure.identifierPhysiologie cellulaire de la synapse [PCS]
dc.contributor.authorHEINE, Martin
hal.structure.identifierPhysiologie cellulaire de la synapse [PCS]
dc.contributor.authorGROC, Laurent
hal.structure.identifierPhysiologie cellulaire de la synapse [PCS]
dc.contributor.authorCHOQUET, Daniel
hal.structure.identifierCentre de physique moléculaire optique et hertzienne [CPMOH]
dc.contributor.authorCOGNET, Laurent
hal.structure.identifierCentre de physique moléculaire optique et hertzienne [CPMOH]
dc.contributor.authorLOUNIS, Brahim
dc.date.issued2006-12-15
dc.identifier.issn0006-3495
dc.description.abstractEnTracking individual nano-objets in live cells during arbitrary long times is an ubiquitous need in modern biology. We present here a method for tracking individual 5 nm gold nanoparticles on live cells. It relies on the photothermal effect and the detection of the Laser Induced Scattering around a NanoAbsorber (LISNA). The key point for recording trajectories at video rate is the use of a triangulation procedure. The effectiveness of the method is tested against Single fluorescent Molecule Tracking in live COS7 cells on subsecond time scales. We further demonstrate recordings for several minutes of AMPA receptors trajectories on the plasma membrane of live neurons. SNaPT has the unique potential to record arbitrary long trajectory of membrane proteins using non-fluorescent nanometer sized labels.
dc.language.isoen
dc.publisherBiophysical Society
dc.subject.ensingle molecule
dc.subject.ensingle particle tracking
dc.subject.enphotothermal
dc.title.enSingle NanoParticle Photothermal Tracking (SNaPT) of 5 nm gold beads in live cells
dc.typeArticle de revue
dc.identifier.doi10.1529/biophysj.106.089771
dc.subject.halPhysique [physics]/Physique [physics]/Optique [physics.optics]
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
dc.identifier.arxiv0704.3809
bordeaux.journalBiophysical Journal
bordeaux.page4598
bordeaux.volume91
bordeaux.issue12
bordeaux.peerReviewedoui
hal.identifierhal-00143902
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00143902v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biophysical%20Journal&rft.date=2006-12-15&rft.volume=91&rft.issue=12&rft.spage=4598&rft.epage=4598&rft.eissn=0006-3495&rft.issn=0006-3495&rft.au=LASNE,%20David&A.%20BLAB,%20Gerhard&BERCIAUD,%20St%C3%A9phane&HEINE,%20Martin&GROC,%20Laurent&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée