LASNE, David; A. BLAB, Gerhard; BERCIAUD, Stéphane; HEINE, Martin; GROC, Laurent; CHOQUET, Daniel; COGNET, Laurent; LOUNIS, Brahim

doi:10.1529/biophysj.106.089771

Metadatos

Mostrar el registro completo del ítem

Licencia de uso del documento

LASNE, David
Centre de physique moléculaire optique et hertzienne [CPMOH]

A. BLAB, Gerhard
Centre de physique moléculaire optique et hertzienne [CPMOH]

BERCIAUD, Stéphane
Centre de physique moléculaire optique et hertzienne [CPMOH]

Idioma

Article de revue

Este ítem está publicado en

Biophysical Journal. 2006-12-15, vol. 91, n° 12, p. 4598

Biophysical Society

Resumen en inglés

Tracking individual nano-objets in live cells during arbitrary long times is an ubiquitous need in modern biology. We present here a method for tracking individual 5 nm gold nanoparticles on live cells. It relies on the photothermal effect and the detection of the Laser Induced Scattering around a NanoAbsorber (LISNA). The key point for recording trajectories at video rate is the use of a triangulation procedure. The effectiveness of the method is tested against Single fluorescent Molecule Tracking in live COS7 cells on subsecond time scales. We further demonstrate recordings for several minutes of AMPA receptors trajectories on the plasma membrane of live neurons. SNaPT has the unique potential to record arbitrary long trajectory of membrane proteins using non-fluorescent nanometer sized labels.< Leer menos

Palabras clave en inglés

single molecule

single particle tracking

photothermal

Metadatos

Licencia de uso del documento

Single NanoParticle Photothermal Tracking (SNaPT) of 5 nm gold beads in live cells

Idioma

Este ítem está publicado en

Resumen en inglés

Palabras clave en inglés

DOI

Orígen

Centros de investigación