Afficher la notice abrégée

Development of an Immunosensor Based on Layered Double Hydroxides for MMR Cancer Biomarker Detection.

hal.structure.identifierInstitut National de Recherche et d'Analyse Physico-Chimique [INRAP]
dc.contributor.authorHAMMAMI, M
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
hal.structure.identifierLaboratoire de Microélectronique et Instrumentations [Monastir]
hal.structure.identifierInstitut National de Recherche et d'Analyse Physico-Chimique [INRAP]
dc.contributor.authorSOUSSOU, A
hal.structure.identifierLaboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules [LR11IPT08]
dc.contributor.authorIDOUDI, F
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorCOHEN-BOUHACINA, T
hal.structure.identifierLaboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules [LR11IPT08]
dc.contributor.authorBOUHAOUALA-ZAHAR, B
hal.structure.identifierInstitut National de Recherche et d'Analyse Physico-Chimique [INRAP]
dc.contributor.authorBACCAR, Z M
dc.date.created2015-04-03
dc.date.issued2015
dc.identifier.issn1536-1241
dc.description.abstractEnAs a potential biomarker for the investigation of cancer inflammatory profiles, macrophage mannose receptor (MMR, CD206) is herein selected to develop an immunosensor based on layered double hydroxide (LDH). Like an endocyte C-type lectin receptor, MMR plays an important role in immune homeostasis by scavenging unwanted mannose glycoproteins. It attracts a progressive attention thanks to its particularly high expression within the tumor microenvironment. There is a great of interest to develop an immunosensor based on an antibody specific to MMR for detection of stroma versus tumor cells. In this work, we studied the feasibility of high sensitive MMR cancer Screen Printed Electrode (SPE) immunosensor. Working electrode of commercialized SPE was modified by immobilization of specific antibody (anti-MMR) into thin layer of LDH nanomaterials. Structural, morphological, and surface properties of LDHs were studied by X-Ray diffraction, atomic force microscopy and Infrared spectroscopy in ATR. Cyclic Voltammetry technique was used to study interaction between the human recombinant MMR protein (rHu-MMR, NSO derived) and an immobilized antibody into developed immunosensor. High specific response of -11.72 μA/ng.mL(-1) (with a correlation coefficient of R(2)=0.994 ) were obtained in linear range of 0.05 ng/mL to 10.0 ng/mL of specific recombinant antigen. The limit of detection (LOD) was less than 15.0 pg/mL. From these attractive results, the feasibility of an electrochemical immunosensor for cancer was proved. Additional experiments to study stability and reproducibility the immunosensor should be completed in perspective to use these anti-MMR based immunosensors for sensing human MMR in patient biopsies and sera.
dc.language.isoen
dc.publisherInstitute of Electrical and Electronics Engineers
dc.subject.enMacrophage mannose receptor
dc.subject.enNanomaterials
dc.subject.enImmunosensor
dc.subject.enLayered Double Hydroxide
dc.title.enDevelopment of an Immunosensor Based on Layered Double Hydroxides for MMR Cancer Biomarker Detection.
dc.typeArticle de revue
dc.identifier.doi10.1109/TNB.2015.2462025
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
bordeaux.journalIEEE Transactions on NanoBioscience
bordeaux.page688-693
bordeaux.volume14
bordeaux.issue7
bordeaux.peerReviewedoui
hal.identifierhal-01255442
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01255442v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=IEEE%20Transactions%20on%20NanoBioscience&rft.date=2015&rft.volume=14&rft.issue=7&rft.spage=688-693&rft.epage=688-693&rft.eissn=1536-1241&rft.issn=1536-1241&rft.au=HAMMAMI,%20M&SOUSSOU,%20A&IDOUDI,%20F&COHEN-BOUHACINA,%20T&BOUHAOUALA-ZAHAR,%20B&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée