| hal.structure.identifier | Institut des Sciences Analytiques [ISA] |  | 
| hal.structure.identifier | Laboratoire des Interfaces et Matériaux Avancés [Monastir] [LIMA] |  | 
| dc.contributor.author | TEKAYA, Nadèje |  | 
| hal.structure.identifier | Laboratoire de l'intégration, du matériau au système [IMS] |  | 
| dc.contributor.author | GAMMOUDI, Ibtissèm |  | 
| hal.structure.identifier | Laboratoire des Interfaces et Matériaux Avancés [Monastir] [LIMA] |  | 
| hal.structure.identifier | Institut des Sciences Analytiques [ISA] |  | 
| dc.contributor.author | BRAIEK, Mohamed |  | 
| hal.structure.identifier | Laboratoire de l'intégration, du matériau au système [IMS] |  | 
| dc.contributor.author | TARBAGUE, Hakim |  | 
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] |  | 
| hal.structure.identifier | Laboratoire des Interfaces et Matériaux Avancés [Monastir] [LIMA] |  | 
| dc.contributor.author | MOROTE, Fabien |  | 
| hal.structure.identifier | Laboratoire de l'intégration, du matériau au système [IMS] |  | 
| dc.contributor.author | RAIMBAULT, Vincent |  | 
| hal.structure.identifier | Laboratoire des Interfaces et Matériaux Avancés [Monastir] [LIMA] |  | 
| dc.contributor.author | SAKLY, Nawfel |  | 
| hal.structure.identifier | Laboratoire de l'intégration, du matériau au système [IMS] |  | 
| dc.contributor.author | REBIÈRE, Dominique |  | 
| hal.structure.identifier | Institut National des Sciences et Technologies de la Mer [Salammbô] [INSTM] |  | 
| dc.contributor.author | BEN OUADA, Hatem |  | 
| hal.structure.identifier | Institut des Sciences Analytiques [ISA] |  | 
| dc.contributor.author | LAGARDE, Florence |  | 
| hal.structure.identifier | Laboratoire des Interfaces et Matériaux Avancés [Monastir] [LIMA] |  | 
| dc.contributor.author | BEN OUADA, Hafedh |  | 
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] |  | 
| dc.contributor.author | COHEN-BOUHACINA, Touria |  | 
| hal.structure.identifier | Laboratoire de l'intégration, du matériau au système [IMS] |  | 
| dc.contributor.author | DÉJOUS, Corinne |  | 
| hal.structure.identifier | Institut des Sciences Analytiques [ISA] |  | 
| dc.contributor.author | JAFFREZIC-RENAULT, Nicole |  | 
| dc.date.created | 2013-04-22 |  | 
| dc.date.issued | 2013-09 |  | 
| dc.identifier.issn | 2213-3437 |  | 
| dc.description.abstractEn | This study examines a biofilm of Arthrospira platensis and its interactions with cadmium and mercury, using electrochemical admittance spectroscopy technique combined with highly sensitive Love wave platform for the real-time detection in liquid medium. Spirulina cells were immobilized via multilayers of polyelectrolyte (PEM) on Si/SiO2 surface of both transducers and characterized using atomic force microscopy (AFM). Scanning electron microscopy (SEM) cell images revealed a first defense mechanism against cadmium at 10−12 M and it immediately takes place after 4 s from injection. The cyanobacteria biofilm becomes more conductive, due to an increase of polyphosphate bodies. An increase of density induces a decrease of frequency. Response time τ90% of the biofilm toward Cd2+ was between 6 and 8 min, while it did not exceed a few seconds toward Hg2+ at 10−12 M. However, the initial rapid stage of mercury adsorption took 40 s to reach the saturated stage. Once external sorption reached the saturated stage, internal mercury uptake began; cations were transported across the cell membrane into the cytoplasm and a beta-HgS precipitation took place, inducing conductivity biofilm decrease, and generating an increase of density, and thus a frequency decrease. SEM images revealed the beginning cell damage at 10−06 M of cadmium and mercury. |  | 
| dc.description.sponsorship | Immunocapteur à ondes de Love ultra-sensible pour la détection rapide de micro-organismes dans l'eau, visant la réalisation d'un dispositif d'alerte - ANR-06-ECOT-0004 |  | 
| dc.language.iso | en |  | 
| dc.publisher | Elsevier |  | 
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/ |  | 
| dc.subject.en | Spirulina |  | 
| dc.subject.en | Heavy metals |  | 
| dc.subject.en | Admittance |  | 
| dc.subject.en | Love wave |  | 
| dc.subject.en | SEM |  | 
| dc.subject.en | AFM |  | 
| dc.title.en | Acoustic, electrochemical and microscopic characterization of interaction of Arthrospira platensis biofilm and heavy metal ions |  | 
| dc.type | Article de revue |  | 
| dc.identifier.doi | 10.1016/j.jece.2013.07.006 |  | 
| dc.subject.hal | Chimie/Chimie analytique |  | 
| dc.subject.hal | Chimie/Génie chimique |  | 
| dc.subject.hal | Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph] |  | 
| bordeaux.journal | Journal of Environmental Chemical Engineering |  | 
| bordeaux.page | 609-619 |  | 
| bordeaux.volume | 1 |  | 
| bordeaux.issue | 3 |  | 
| bordeaux.peerReviewed | oui |  | 
| hal.identifier | hal-00878629 |  | 
| hal.version | 1 |  | 
| hal.popular | non |  | 
| hal.audience | Internationale |  | 
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-00878629v1 |  | 
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Environmental%20Chemical%20Engineering&rft.date=2013-09&rft.volume=1&rft.issue=3&rft.spage=609-619&rft.epage=609-619&rft.eissn=2213-3437&rft.issn=2213-3437&rft.au=TEKAYA,%20Nad%C3%A8je&GAMMOUDI,%20Ibtiss%C3%A8m&BRAIEK,%20Mohamed&TARBAGUE,%20Hakim&MOROTE,%20Fabien&rft.genre=article |  |