Afficher la notice abrégée

Rationalisation of a mechanism for sensing single point variants in target DNA using anthracene-tagged base discriminating probes

hal.structure.identifierUniversity of Birmingham [Birmingham]
dc.contributor.authorDUPREY, Jean Louis
hal.structure.identifierInstitut des Sciences Moléculaires [ISM]
dc.contributor.authorBASSANI, Dario M.
hal.structure.identifierUniversity of Birmingham [Birmingham]
dc.contributor.authorHYDE, Eva I.
hal.structure.identifierLaboratoire Ondes et Matière d'Aquitaine [LOMA]
dc.contributor.authorJONUSAUSKAS, Gediminas
hal.structure.identifierUniversity of Birmingham [Birmingham]
dc.contributor.authorLUDWIG, Christian
hal.structure.identifierMacquarie University
dc.contributor.authorRODGER, Alison
hal.structure.identifierUniversity of Birmingham [Birmingham]
dc.contributor.authorSPENCER, Neil
hal.structure.identifierQueen's University [Belfast] [QUB]
dc.contributor.authorVYLE, Joseph
hal.structure.identifierUniversity of Birmingham [Birmingham]
dc.contributor.authorWILKIE, John
hal.structure.identifierUniversity of Birmingham [Birmingham]
dc.contributor.authorZHAO, Zheng-Yun
hal.structure.identifierUniversity of Birmingham [Birmingham]
dc.contributor.authorTUCKER, James H. R.
dc.date.created2018-07-17
dc.date.issued2018-09-21
dc.identifier.issn1477-0520
dc.description.abstractEnThe labelling of DNA oligonucleotides with signalling groups that give a unique response to duplex formation depending on the target sequence is a highly effective strategy in the design of DNA-based hybridisation sensors. A key challenge in the design of these so-called base discriminating probes (BDPs) is to understand how the local environment of the signalling group affects the sensing response. The work herein describes a comprehensive study involving a variety of photophysical techniques, NMR studies and molecular dynamics simulations, on anthracene-tagged oligonucleotide probes that can sense single base changes (point variants) in target DNA strands. A detailed analysis of the fluorescence sensing mechanism is provided, with a particular focus on rationalising the high dependence of this process on not only the linker stereochemistry but also the site of nucleobase variation within the target strand. The work highlights the various factors and techniques used to respectively underpin and rational-ise the BDP approach to point variant sensing, which relies on different responses to duplex formation rather than different duplex binding strengths.
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enRationalisation of a mechanism for sensing single point variants in target DNA using anthracene-tagged base discriminating probes
dc.typeArticle de revue
dc.identifier.doi10.1039/c8ob01710g
dc.subject.halPhysique [physics]/Physique [physics]/Chimie-Physique [physics.chem-ph]
bordeaux.journalOrganic & Biomolecular Chemistry
bordeaux.page6576-6585
bordeaux.volume16
bordeaux.issue35
bordeaux.peerReviewedoui
hal.identifierhal-01953231
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01953231v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Organic%20&%20Biomolecular%20Chemistry&rft.date=2018-09-21&rft.volume=16&rft.issue=35&rft.spage=6576-6585&rft.epage=6576-6585&rft.eissn=1477-0520&rft.issn=1477-0520&rft.au=DUPREY,%20Jean%20Louis&BASSANI,%20Dario%20M.&HYDE,%20Eva%20I.&JONUSAUSKAS,%20Gediminas&LUDWIG,%20Christian&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée