Silica nanoparticles-assisted electrochemical biosensor for the rapid, sensitive and specific detection of Escherichia coli
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| hal.structure.identifier | Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB] | |
| dc.contributor.author | MATHELIÉ-GUINLET, Marion | |
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| dc.contributor.author | COHEN-BOUHACINA, Touria | |
| hal.structure.identifier | Laboratoire de l'intégration, du matériau au système [IMS] | |
| dc.contributor.author | GAMMOUDI, Ibtissem | |
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| dc.contributor.author | MARTIN, Axel | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | BEVEN, Laure | |
| hal.structure.identifier | Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB] | |
| dc.contributor.author | DELVILLE, Marie-Hélène | |
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| dc.contributor.author | GRAUBY-HEYWANG, Christine | |
| dc.date.issued | 2019 | |
| dc.identifier.issn | 0925-4005 | |
| dc.description.abstractEn | To face the emerging issues raised by bacterial infections in biomedical, food and environmental contexts, the combination of nanotechnologies and biosensors characteristics is increasingly seen as a promising tool to develop rapid, highly sensitive and specific devices for reliable bacterial detection and quantification. We present here the design of an electrochemical immuno-biosensor, integrating non cytotoxic silica nanoparticles (NPs), in a two-step spin coating process. Unlike biosensors that integrate a unique antibody-based layer without NPs, this new tool allows, through cyclic voltammetry measurements, the continuous and non-saturated detection of E. coli bacteria in only 5 min. and can reach up to 103 CFU/mL in 30 min. It offers great promises for industrial purposes as electrode functionalization is rather simple and its architecture can be tuned on demand to target a variety of micro-organisms, and the highlighted indicators (variations of current intensity, intensity ratios and/or difference of potentials) can easily be implemented in a screen displayed packaging. | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.subject.en | Nanoparticles | |
| dc.subject.en | Immunosensor | |
| dc.subject.en | Cyclic voltammetry | |
| dc.subject.en | E. coli | |
| dc.title.en | Silica nanoparticles-assisted electrochemical biosensor for the rapid, sensitive and specific detection of Escherichia coli | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1016/j.snb.2019.03.144 | |
| dc.subject.hal | Chimie/Autre | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biotechnologies | |
| bordeaux.journal | Sensors and Actuators B: Chemical | |
| bordeaux.page | 314-320 | |
| bordeaux.volume | 292 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-02123016 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02123016v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Sensors%20and%20Actuators%20B:%20Chemical&rft.date=2019&rft.volume=292&rft.spage=314-320&rft.epage=314-320&rft.eissn=0925-4005&rft.issn=0925-4005&rft.au=MATHELI%C3%89-GUINLET,%20Marion&COHEN-BOUHACINA,%20Touria&GAMMOUDI,%20Ibtissem&MARTIN,%20Axel&BEVEN,%20Laure&rft.genre=article |
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