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Cytological, biochemical and molecular events of the embryogenic state in Douglas-fir (Pseudotsuga menziesii [Mirb.])

hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
hal.structure.identifierUniversité de Limoges [UNILIM]
dc.contributor.authorGAUTIER, Florian
hal.structure.identifierLaboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant [PIAF]
dc.contributor.authorLABEL, Philippe
hal.structure.identifierInstitute of Experimental Botany of the Czech Academy of Sciences [IEB / CAS]
dc.contributor.authorELIÁŠOVÁ, Kateřina
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLEPLÉ, Jean-Charles
hal.structure.identifierInstitute of Experimental Botany of the Czech Academy of Sciences [IEB / CAS]
dc.contributor.authorMOTYKA, Václav
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorBOIZOT, Nathalie
hal.structure.identifierInstitute of Experimental Botany of the Czech Academy of Sciences [IEB / CAS]
dc.contributor.authorVONDRAKOVA, Zuzana
hal.structure.identifierInstitute of Experimental Botany of the Czech Academy of Sciences [IEB / CAS]
dc.contributor.authorMALBECK, Jiří
hal.structure.identifierInstitute of Experimental Botany of the Czech Academy of Sciences [IEB / CAS]
dc.contributor.authorTRÁVNÍČKOVÁ, Alena
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorLE METTE, Claire
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorLESAGE-DESCAUSES, Marie-Claude
hal.structure.identifierPlateforme Protéome [Bordeaux]
dc.contributor.authorLOMENECH, Anne-Marie
hal.structure.identifierPôle Biotechnologie et Sylviculture Avancée
dc.contributor.authorTRONTIN, Jean-François
hal.structure.identifierUniversité de Limoges [UNILIM]
dc.contributor.authorCOSTA, Guy
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorLELU-WALTER, Marie-Anne
hal.structure.identifierBiologie intégrée pour la valorisation de la diversité des arbres et de la forêt [BioForA]
dc.contributor.authorTEYSSIER, Caroline
dc.date.issued2019
dc.identifier.issn1664-462X
dc.description.abstractEnSomatic embryogenesis techniques have been developed for most coniferous species, but only using very juvenile material. To extend the techniques' scope, better integrated understanding of the key biological, physiological and molecular characteristics of embryogenic state is required. Therefore, embryonal masses (EMs) and non-embryogenic calli (NECs) have been compared during proliferation at multiple levels. EMs and NECs originating from a single somatic embryo (isogenic lines) of each of three unrelated genotypes were used in the analyses, which included comparison of the lines' anatomy by transmission light microscopy, transcriptomes by RNAseq Illumina sequencing, proteomes by free-gel analysis, contents of endogenous phytohormones (indole-3-acetic acid, cytokinins and ABA) by LC-MS analysis, and soluble sugar contents by HPLC. EMs were characterized by upregulation (relative to levels in NECs) of transcripts, proteins, transcription factors and active cytokinins associated with cell differentiation accompanied by histological, carbohydrate content and genetic markers of cell division. In contrast, NECs were characterized by upregulation (relative to levels in EMs) of transcripts, proteins and products associated with responses to stimuli (ABA, degradation forms of cytokinins, phenols), oxidative stress (reactive oxygen species) and carbohydrate storage (starch). Sub-Network Enrichment Analyses that highlighted functions and interactions of transcripts and proteins that significantly differed between EMs and NECs corroborated these findings. The study shows the utility of a novel approach involving integrated multi-scale transcriptomic, proteomic, biochemical, histological and anatomical analyses to obtain insights into molecular events associated with embryogenesis and more specifically to the embryogenic state of cell in Douglas-fir.
dc.language.isoen
dc.publisherFrontiers
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enCytological, biochemical and molecular events of the embryogenic state in Douglas-fir (Pseudotsuga menziesii [Mirb.])
dc.typeArticle de revue
dc.identifier.doi10.3389/fpls.2019.00118
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences de l'environnement
bordeaux.journalFrontiers in Plant Science
bordeaux.page26 p.
bordeaux.volume10
bordeaux.peerReviewedoui
hal.identifierhal-02626893
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02626893v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Plant%20Science&rft.date=2019&rft.volume=10&rft.spage=26%20p.&rft.epage=26%20p.&rft.eissn=1664-462X&rft.issn=1664-462X&rft.au=GAUTIER,%20Florian&LABEL,%20Philippe&ELI%C3%81%C5%A0OV%C3%81,%20Kate%C5%99ina&LEPL%C3%89,%20Jean-Charles&MOTYKA,%20V%C3%A1clav&rft.genre=article


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