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Multiplex real‐time PCR assays for the detection and identification of Heterobasidion species attacking conifers in Europe

hal.structure.identifierAgence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
dc.contributor.authorIOOS, R.
hal.structure.identifierAgence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
dc.contributor.authorCHRETIEN, P.
hal.structure.identifierAgence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
dc.contributor.authorPERRAULT, J.
hal.structure.identifierAgence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
dc.contributor.authorJEANDEL, C.
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorDUTECH, Christian Cyril
hal.structure.identifierUniversita di Torino
dc.contributor.authorGONTHIER, P.
hal.structure.identifierUniversita di Torino
dc.contributor.authorSILLO, F.
hal.structure.identifierNorsk institutt for bioøkonomi=Norwegian Institute of Bioeconomy Research [NIBIO]
dc.contributor.authorHIETALA, A.M.
hal.structure.identifierNorsk institutt for bioøkonomi=Norwegian Institute of Bioeconomy Research [NIBIO]
dc.contributor.authorSOLHEIM, H.
hal.structure.identifierUniversita di Torino
dc.contributor.authorHUBERT, J.
dc.date.issued2019
dc.identifier.issn0032-0862
dc.description.abstractEnFour species of the destructive forest pathogen Heterobasidion annosum sensu lato (s.l.) are present in Europe: H. annosum sensu stricto (s.s.), H. abietinum and H. parviporum are native species, while H. irregulare is a non-native invasive species currently reported only in Italy, yet recommended for regulation throughout Europe. In this study, real-time PCR detection tests were developed for each of the four species, which can be used simultaneously or individually thanks to probes labelled with species-specific fluorescent dyes. The different performance criteria of each assay were evaluated, and it was determined that they were theoretically capable of detecting amounts of DNA corresponding to 311, 29 and 29 cell nuclei in H. annosum s.s., H. irregulare and H. parviporum, respectively. The specificity of each assay was assessed with a wide set of strains. Real-time PCR tests successfully detected Heterobasidion species from 36 fruiting bodies taken from the forest, as well as from artificially inoculated or naturally infected wood samples. The multiplex real-time PCR assays developed in this study could have practical applications both in forest management and in phytosanitary monitoring.
dc.description.sponsorshipRecherches Avancées sur l'Arbre et les Ecosytèmes Forestiers - ANR-11-LABX-0002
dc.language.isoen
dc.publisherWiley
dc.subject.enconifer root rot
dc.subject.enmolecular assay
dc.subject.enmonitoring
dc.title.enMultiplex real‐time PCR assays for the detection and identification of Heterobasidion species attacking conifers in Europe
dc.typeArticle de revue
dc.identifier.doi10.1111/ppa.13071
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalPlant Pathology
bordeaux.volumeonline first
bordeaux.peerReviewedoui
hal.identifierhal-02627405
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02627405v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Pathology&rft.date=2019&rft.volume=online%20first&rft.eissn=0032-0862&rft.issn=0032-0862&rft.au=IOOS,%20R.&CHRETIEN,%20P.&PERRAULT,%20J.&JEANDEL,%20C.&DUTECH,%20Christian%20Cyril&rft.genre=article


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