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Differential accumulation of soluble proteins in roots of metallicolous and nonmetallicolous populations of Agrostis capillaris L. exposed to Cu

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorHEGO, Elena
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorBES, Clémence
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorBEDON, Franck
hal.structure.identifierSwiss Institute of Bioinformatics [Lausanne] [SIB]
dc.contributor.authorPALAGI, Patricia M.
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorCHAUMEIL, Philippe
hal.structure.identifierUniversité Sciences et Technologies - Bordeaux 1 [UB]
dc.contributor.authorBARRÉ, Aurélien
hal.structure.identifierPlateforme Protéome [Bordeaux]
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierPlateforme Protéome [Bordeaux]
dc.contributor.authorDUPU, Jean-William
hal.structure.identifierPlateforme Protéome [Bordeaux]
dc.contributor.authorBONNEU, Marc
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLALANNE, Céline
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorMENCH, Michel
dc.date.issued2014
dc.identifier.issn1615-9853
dc.description.abstractEnDifferential expression of soluble proteins was explored in roots of metallicolous (M) and non-M (NM) plants of Agrostis capillaris L. exposed to increasing Cu to partially identify molecular mechanisms underlying higher Cu tolerance in M plants. Plants were cultivated for 2 months on perlite with a CuSO4 (1–30 μM) spiked-nutrient solution. Soluble proteins extracted by the trichloroacetic acid/acetone procedure were separated with 2DE (linear 4–7 pH gradient). After Coomassie Blue staining and image analysis, 19 proteins differentially expressed were identified using LC-MS/MS and Expressed Sequence Tag (ESTs) databases. At supra-optimal Cu exposure (15–30 μM), glycolysis was likely altered in NM roots with increased production of glycerone-P and methylglyoxal based on overexpression of triosephosphate isomerase and fructose bisphosphate aldolase. Changes in tubulins and higher expressions of 5-methyltetrahydropteroyltriglutamatehomocysteine methyltransferase and S-adenosylmethionine synthase underpinned impacts on the cytoskeleton and stimulation of ethylene metabolism. Increased l-methionine and S-adenosylmethionine amounts may also facilitate production of nicotianamine, which complexes Cu, and of l-cysteine, needed for metallothioneins and GSH. In M roots, the increase of [Cu/Zn] superoxide dismutase suggested a better detoxification of superoxide, when Cu exposure rose. Higher Cu-tolerance of M plants would rather result from simultaneous cooperation of various processes than from a specific mechanism.
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.subjectCu-tolerance
dc.subjectplant proteomics
dc.subjectsuperoxide dismutase
dc.subject.enagrostis
dc.title.enDifferential accumulation of soluble proteins in roots of metallicolous and nonmetallicolous populations of Agrostis capillaris L. exposed to Cu
dc.typeArticle de revue
dc.identifier.doi10.1002/pmic.201300168
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalProteomics
bordeaux.page1746-1758
bordeaux.volume14
bordeaux.issue15
bordeaux.peerReviewedoui
hal.identifierhal-02633974
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02633974v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Proteomics&rft.date=2014&rft.volume=14&rft.issue=15&rft.spage=1746-1758&rft.epage=1746-1758&rft.eissn=1615-9853&rft.issn=1615-9853&rft.au=HEGO,%20Elena&BES,%20Cl%C3%A9mence&BEDON,%20Franck&PALAGI,%20Patricia%20M.&CHAUMEIL,%20Philippe&rft.genre=article


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