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Copper stress-induced changes in leaf soluble proteome of Cu-sensitive and tolerant Agrostis capillaris L. populations

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorHEGO, Elena
hal.structure.identifierBordeaux INP
dc.contributor.authorVILAIN, Sebastien
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorBARRÉ, Aurélien
hal.structure.identifierCentre Génomique Fonctionnelle, Plateforme Protéome
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierCentre Génomique Fonctionnelle, Plateforme Protéome
dc.contributor.authorDUPUY, Jean-William
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLALANNE, Céline
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorBONNEU, Marc
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorMENCH, Michel
dc.date.issued2016
dc.identifier.issn1615-9853
dc.description.abstractEnChanges in leaf soluble proteome were explored in 3-month-old plants of metallicolous (M) and non-metallicolous (NM) Agrostis capillaris L. populations exposed to increasing Cu concentrations (1-50 μM), to investigate molecular mechanisms underlying plant responses to Cu excess and tolerance of M plants. Plants were cultivated on perlite (CuSO4 spiked-nutrient solution). Soluble proteins, extracted by the trichloroacetic acid/acetone procedure, were separated with 2-DE (linear 4–7 pH gradient). Analysis of CCB-stained gels (PDQuest) reproducibly detected 214 spots, and 64 proteins differentially expressed were identified using LC-MS/MS. In both populations, Cu excess impacted both light-dependent (OEE, cytochrome b6-f complex and chlorophyll a-b binding protein), and -independent (RuBisCO) photosynthesis reactions, more intensively in NM leaves (ferredoxin-NADP reductase and metalloprotease FTSH2). In both populations, up-regulation of isocitrate dehydrogenase and cysteine/methionine synthases respectively suggested increased isocitrate oxidation and enhanced need for S-containing amino-acids, likely for chelation and detoxification. In NM leaves, an increasing need for energetic compounds was indicated by the stimulation of ATPases, glycolysis, pentose phosphate pathway and Calvin cycle enzymes; impacts on protein metabolism and oxidative stress increase were respectively suggested by the rise of chaperones and redox enzymes. Overexpression of a HSP70 may be pivotal for M Cu tolerance by protecting protein metabolism.
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.subjectplant proteomics
dc.subject.enheat-shock protein
dc.subject.enmass spectrometry
dc.subject.enLC-MS/MS
dc.subject.enoxidative stress
dc.subject.enphotosynthesis
dc.title.enCopper stress-induced changes in leaf soluble proteome of Cu-sensitive and tolerant Agrostis capillaris L. populations
dc.typeArticle de revue
dc.identifier.doi10.1002/pmic.201500083
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalProteomics
bordeaux.page1386-1397
bordeaux.volume16
bordeaux.issue9
bordeaux.peerReviewedoui
hal.identifierhal-02640090
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02640090v1
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