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Allelic variation at the rpv1 locus controls partial resistance to Plum pox virus infection in Arabidopsis thaliana

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPOQUE, Sylvain
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPAGNY, Gaëlle
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorOUIBRAHIM, Laurence
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCHAGUE, Aurélie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorEYQUARD, Jean-Philippe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCABALLERO, Mélodie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorCARANTA, Carole
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierfrom patterns to models in computational biodiversity and biotechnology [PLEIADE]
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorMARIETTE, Stéphanie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDECROOCQ, Véronique
dc.date.issued2015-06-25
dc.identifier.issn1471-2229
dc.description.abstractEnSharka is caused by Plum pox virus (PPV) in stone fruit trees. In orchards, the virus is transmitted by aphids and by grafting. In Arabidopsis, PPV is transferred by mechanical inoculation, by biolistics and by agroinoculation with infectious cDNA clones. Partial resistance to PPV has been observed in the Cvi-1 and Col-0 Arabidopsis accessions and is characterized by a tendency to escape systemic infection. Indeed, only one third of the plants are infected following inoculation, in comparison with the susceptible Ler accession. Genetic analysis showed this partial resistance to be monogenic or digenic depending on the allelic configuration and recessive. It is detected when inoculating mechanically but is overcome when using biolistic or agroinoculation. A genome-wide association analysis was performed using multiparental lines and 147 Arabidopsis accessions. It identified a major genomic region, rpv1. Fine mapping led to the positioning of rpv1 to a 200 kb interval on the long arm of chromosome 1. A candidate gene approach identified the chloroplast phosphoglycerate kinase (cPGK2) as a potential gene underlying the resistance. A virus-induced gene silencing strategy was used to knock-down cPGK2 expression, resulting in drastically reduced PPV accumulation. These results indicate that rpv1 resistance to PPV carried by the Cvi-1 and Col-0 accessions is linked to allelic variations at the Arabidopsis cPGK2 locus, leading to incomplete, compatible interaction with the virus.
dc.language.isoen
dc.publisherBioMed Central
dc.subjectPPV
dc.subject.enpartial resistance
dc.subject.enrecessive resistance
dc.subject.enQTL mapping
dc.subject.enassociation mapping
dc.subject.enPlum pox virus
dc.subject.enArabidopsis thaliana
dc.subject.encPGK
dc.title.enAllelic variation at the rpv1 locus controls partial resistance to Plum pox virus infection in Arabidopsis thaliana
dc.typeArticle de revue
dc.identifier.doi10.1186/s12870-015-0559-5
dc.subject.halSciences du Vivant [q-bio]/Biodiversité
dc.description.sponsorshipEuropeSharka Containment
bordeaux.journalBMC Plant Biology
bordeaux.page159
bordeaux.volume15
bordeaux.issue159
bordeaux.peerReviewedoui
hal.identifierhal-01262384
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01262384v1
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