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Development, mapping and transferability of Fragaria EST-SSRs within the Rosodae supertribe

hal.structure.identifierUnité de recherches Espèces Fruitières et Vigne [UREFV]
dc.contributor.authorROUSSEAU-GUEUTIN, Mathieu
hal.structure.identifierUnité de recherches Espèces Fruitières et Vigne [UREFV]
dc.contributor.authorRICHARD, Laurent
hal.structure.identifierUnité de recherches Espèces Fruitières et Vigne [UREFV]
dc.contributor.authorLE DANTEC, Loick
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorCARON, Henri
hal.structure.identifierUnité de recherches Espèces Fruitières et Vigne [UREFV]
dc.contributor.authorDENOYES-ROTHAN, Beatrice
dc.date.issued2011
dc.identifier.issn0179-9541
dc.description.abstractEnThe Rosodae supertribe is composed of economically important species (strawberry, rose and raspberry). In order to extend the repertoire of markers for optimizing genetic mapping and synteny within this supertribe, we developed 20 functional markers from two Fragaria cDNA libraries enriched in AG/AC microsatellite motifs. We tested their transferability within the Rosodae supertribe, including two reference diploid and octoploid strawberry segregating populations. Out of these 20 functional markers, 70% revealed polymorphism in both diploid and octoploid Fragaria segregating populations and were mapped on the diploid genetic map to provide useful information for further marker-assisted selection. These 20 EST-SSRs were also tested in 17 wild Fragaria species and displayed a high cross-species transferability (mean of 95%), providing new markers for further diversity studies within the genus. These markers were also transferable to the phylogenetically close Potentilla genus, whereas their transferability was lower for the polyploid Duchesnea indica species and for the more distant Rosa genus. Their use for genetic mapping within the Rosodae will further permit a comparison of genetic information among the different genera.
dc.language.isoen
dc.publisherWiley
dc.subjectFRAISIER
dc.subjectCARTOGRAPHIE GENETIQUE
dc.subject.enGENIC MICROSATELLITES
dc.subject.enFUNCTIONAL MARKERS
dc.subject.enMARKER TRANSFERABILITY
dc.subject.enROSODAE
dc.subject.enPOLYMORPHISM
dc.subject.enSTRAWBERRY
dc.subject.enFRAGARIA
dc.title.enDevelopment, mapping and transferability of Fragaria EST-SSRs within the Rosodae supertribe
dc.typeArticle de revue
dc.identifier.doi10.1111/j.1439-0523.2010.01785.x
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant Breeding
bordeaux.page248-255
bordeaux.volume130
bordeaux.issue2
bordeaux.peerReviewedoui
hal.identifierhal-02646384
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02646384v1
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