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DNA barcoding in the rust genus Chrysomyxa and its implications for the phylogeny of the genus

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierNatural Resources Canada [NRCan]
dc.contributor.authorFEAU, Nicolas
hal.structure.identifierNatural Resources Canada [NRCan]
hal.structure.identifierCentre d’étude de la forêt
dc.contributor.authorVIALLE, Agathe
hal.structure.identifierNatural Resources Canada [NRCan]
dc.contributor.authorALLAIRE, Mathieu
hal.structure.identifierRuhr University Bochum = Ruhr-Universität Bochum [RUB]
dc.contributor.authorMAIER, Wolfgang
hal.structure.identifierNatural Resources Canada [NRCan]
hal.structure.identifierUniversity of British Columbia [UBC]
dc.contributor.authorHAMELIN, Richard C.
dc.date.issued2011
dc.identifier.issn0027-5514
dc.description.abstractEnChrysomyxa rusts are fungal pathogens widely present in the boreal forest. Taxonomic delimitation and precise species identification are difficult within this genus because several species display similar morphological features. We applied a DNA barcode system based on the ribosomal internal transcribed spacer region (ITS), large subunit (28S) ribosomal RNA gene, mitochondrial cytochrome oxidase 1 (CO1) and mitochondrial NADH dehydrogenase subunit 6 (NAD6) in 86 strains from 16 different Chrysomyxa species, including members of the Chrysomyxa ledi species complex. The nuclear ITS and 28S loci revealed higher resolving power than the mitochondrial genes. Amplification of the full CO1 barcode region failed due to the presence of introns limiting the dataset obtained with this barcode. In most cases the ITS barcodes were in agreement with taxonomic species based on phenotypic characters. Nevertheless we observed genetically distinct (different DNA barcodes) lineages within Chrysomyxa pyrolae and Chrysomyxa rhododendri, providing some evidence for allopatric speciation within these morphologically defined species. This finding, together with the observed pattern of host specificities of the studied rust fungi, suggest that species diversification within the C. ledi species complex might be governed by a set of factors such as specialisation to certain Ericaceae species as telial hosts and to a lesser extent specialization to different spruce species as aecial hosts. Moreover allopatric speciation by geographic disruption of species also seems to take place. When our data were integrated into a broader phylogenetic framework the Chrysomyxa genus unexpectedly was not resolved as a monophyletic group. Indeed the spruce cone rusts C. pyrolae and C. monesis coalesced with the pine needle rusts belonging to the genus Coleosporium, whereas the microcyclic species Chrysomyxa weirii was embedded within a clade comprising the genus Melampsora.
dc.language.isoen
dc.publisherMycological Society of America
dc.subjectDNA barcode
dc.subjectmycology
dc.subject.enchrysomyxa needle rusts
dc.subject.encone rusts
dc.subject.enfungal pathogen
dc.subject.enmitochondrial heteroplasmon
dc.subject.enphylogeny
dc.subject.enspecies complex
dc.title.enDNA barcoding in the rust genus Chrysomyxa and its implications for the phylogeny of the genus
dc.typeArticle de revue
dc.identifier.doi10.3852/10-426
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Mycologie
bordeaux.journalMycologia
bordeaux.page1250-1266
bordeaux.volume103
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-02647509
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647509v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Mycologia&rft.date=2011&rft.volume=103&rft.issue=6&rft.spage=1250-1266&rft.epage=1250-1266&rft.eissn=0027-5514&rft.issn=0027-5514&rft.au=FEAU,%20Nicolas&VIALLE,%20Agathe&ALLAIRE,%20Mathieu&MAIER,%20Wolfgang&HAMELIN,%20Richard%20C.&rft.genre=article


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