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Proteomic plasticity of two Eucalyptus genotypes under contrasted water regimes in the field

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorBEDON, Franck
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierCentre de Coopération Internationale en Recherche Agronomique pour le Développement [Cirad]
hal.structure.identifierCentre de Recherche sur la Durabilité et la Productivité des Plantations Industrielles [CRDPI]
dc.contributor.authorVILLAR, Emilie
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorVINCENT, Delphine
hal.structure.identifierUniversité Sciences et Technologies - Bordeaux 1 [UB]
dc.contributor.authorDUPUY, Jean-William
hal.structure.identifierUniversité Sciences et Technologies - Bordeaux 1 [UB]
dc.contributor.authorLOMENECH, Anne-Marie
hal.structure.identifierCentre de Recherche sur la Durabilité et la Productivité des Plantations Industrielles [CRDPI]
dc.contributor.authorMABIALANGOMA, André
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorCHAUMEIL, Philippe
hal.structure.identifierUniversité Sciences et Technologies - Bordeaux 1 [UB]
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorBARRÉ, Aurélien
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierCentre de Coopération Internationale en Recherche Agronomique pour le Développement [Cirad]
dc.contributor.authorGION, Jean-Marc
dc.date.issued2012
dc.identifier.issn0140-7791
dc.description.abstractEnWater deficit affects tree growth and limits wood production. In an attempt to identify the molecular triggers of adaptation mechanisms to water deficit in Eucalyptus, we investigated protein expression patterns of two ecophysiologically contrasted Eucalyptus genotypes. They were grown in the field in either natural conditions or irrigated for 7 weeks during the dry season in the Republic of Congo. At the phenotypic level, genotype (G), treatment (T) and/or G × T interaction effects were observed for above- and below-ground biomass-related traits. At the molecular level, changes in protein abundance were recorded in leaves (acidic pH 4–7, and basic pH 7–11, proteomes) and stems (acidic proteome) using two-dimensional gel electrophoresis (2-DE). One third of the detected protein spots displayed significant G, T and/or G × T effects, and 158 of them were identified by tandem mass spectrometry (LC-MS/MS) analysis. Thus, several proteins whose molecular plasticity was genetically controlled (i.e. G × T effect) were revealed, highlighting adaptive mechanisms to water deficit specific to each genotype, namely cell wall modification, cell detoxification and osmoregulation. Transcript abundances corresponding to G × T proteins were also investigated by quantitative RT-PCR. These proteins represent relevant targets to improve drought resistance in this ecologically and economically important forest tree genus.
dc.language.isoen
dc.publisherWiley
dc.subjectélectrophorèse sur gel à deux dimensions
dc.subject.enadaptation
dc.subject.enproteome
dc.subject.enmolecular plasticity
dc.subject.entwo-dimensional gel electrophoresis (2-DE)
dc.subject.enwater deficit
dc.title.enProteomic plasticity of two Eucalyptus genotypes under contrasted water regimes in the field
dc.typeArticle de revue
dc.identifier.doi10.1111/j.1365-3040.2011.02452.x
dc.subject.halSciences du Vivant [q-bio]/Ecologie, Environnement
bordeaux.journalPlant, Cell and Environment
bordeaux.page790-805
bordeaux.volume35
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-02647900
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647900v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant,%20Cell%20and%20Environment&rft.date=2012&rft.volume=35&rft.issue=4&rft.spage=790-805&rft.epage=790-805&rft.eissn=0140-7791&rft.issn=0140-7791&rft.au=BEDON,%20Franck&VILLAR,%20Emilie&VINCENT,%20Delphine&DUPUY,%20Jean-William&LOMENECH,%20Anne-Marie&rft.genre=article


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