High throughput microsatellite genotyping in oak species
| hal.structure.identifier | Biodiversité, Gènes et Ecosystèmes [BioGeCo] | |
| dc.contributor.author | LEPAIS, Olivier | |
| hal.structure.identifier | Biodiversité, Gènes et Ecosystèmes [BioGeCo] | |
| dc.contributor.author | LÉGER, Valérie | |
| hal.structure.identifier | Biodiversité, Gènes et Ecosystèmes [BioGeCo] | |
| dc.contributor.author | GERBER, Sophie | |
| dc.date.issued | 2006 | |
| dc.identifier.issn | 0037-5349 | |
| dc.description.abstractEn | Microsatellites are widely used markers for multiple purposes in oaks. We describe a complete procedure for cheap DNA extraction and fast microsatellites genotyping by multiplex PCR. 10 loci were selected to form two multiplex kits including three loci that show a high differentiation between Quercus robur and Q. petraea. The loci were tested in three oak species and show a high mean genetic diversity of 0.84. The cumulative exclusion probability for parentage analysis was 0.999977 for single parent and 1.0 for paternity. Finally, the relatively high differentiation coefficient (Gst = 0.04) will facilitate species assignment based on genotypes in oaks | |
| dc.language.iso | en | |
| dc.publisher | Walter De Gruyter | |
| dc.subject | MICROSATELLITES | |
| dc.subject.en | MULTIPLEX PCR | |
| dc.subject.en | GENETIC ASSIGNMENT | |
| dc.subject.en | PARENTAGE ANALYSIS | |
| dc.subject.en | CHENE | |
| dc.title.en | High throughput microsatellite genotyping in oak species | |
| dc.type | Article de revue | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Sciences agricoles/Sylviculture, foresterie | |
| bordeaux.journal | Silvae Genetica | |
| bordeaux.page | 238-240 | |
| bordeaux.volume | 55 | |
| bordeaux.issue | 4-5 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-02661668 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02661668v1 | |
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