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Protein polymorphism between two Picea abies ecotypes as revealed by two-dimensional gel electrophoresis and tandem mass spectrometry

hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorVALCU, Cristina-Maria
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLALANNE, Céline
hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorMÜLLER-STARCK, Gerhard
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
hal.structure.identifierTechnische Universität Munchen - Technical University Munich - Université Technique de Munich [TUM]
dc.contributor.authorSCHLINK, Katja
dc.date.issued2008
dc.identifier.issn0022-1503
dc.description.abstractEnIn species with high gene flow and consequent low interpopulation differentiation over wide geographic ranges, differential gene expression along ecological gradients often reveals adaptive significance. We investigated potential differences in protein expression between Picea abies ecotypes adapted to contrasting altitude conditions. Protein expression patterns were compared between needles and roots of 2-month-old P. abies seedlings by means of 2-dimensional electrophoresis. Proteins exhibiting differential expression between the 2 ecotypes were analyzed by tandem mass spectrometry. A total of 19 proteins exhibited qualitative or quantitative polymorphism between the 2 populations. These proteins exhibited organ-specific expression, and the level of interpopulation protein polymorphism was organ dependent. Among differentially expressed proteins, we identified proteins involved in photosynthesis, photorespiration, root tracheary element differentiation, and transmitochondrial membrane transport. Our results show that P. abies seedlings from locally adapted ecotypes exhibit consistent differences in protein expression. The expression polymorphism of some of these proteins has potential adaptive significance.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subjectEPICEA COMMUN
dc.title.enProtein polymorphism between two Picea abies ecotypes as revealed by two-dimensional gel electrophoresis and tandem mass spectrometry
dc.typeArticle de revue
dc.identifier.doi10.1093/jhered/esn007
dc.subject.halSciences du Vivant [q-bio]/Génétique
bordeaux.journalJournal of Heredity
bordeaux.page364-375
bordeaux.volume99
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-02665815
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02665815v1
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