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Sampling tissue for DNA analysis of trees: Trunk cambium as an alternative to canopy leaves

hal.structure.identifierUniversiteit Gent = Ghent University = Université de Gand [UGENT]
dc.contributor.authorCOLPAERT, N.
hal.structure.identifierNatural Environment Research Council [NERC]
dc.contributor.authorCAVERS, Stephen
hal.structure.identifierBiodiversité, Gènes et Ecosystèmes [BioGeCo]
dc.contributor.authorBANDOU, Eric
hal.structure.identifierNatural Environment Research Council [NERC]
dc.contributor.authorCARON, Henri
hal.structure.identifierUniversiteit Gent = Ghent University = Université de Gand [UGENT]
dc.contributor.authorGHEYSEN, Godelieve
hal.structure.identifierNatural Environment Research Council [NERC]
hal.structure.identifierThe University of Queensland [UQ [All campuses : Brisbane, Dutton Park Gatton, Herston, St Lucia and other locations]]
dc.contributor.authorLOWE, Johannes
dc.date.issued2005
dc.identifier.issn0037-5349
dc.description.abstractEnThe number of studies of tropical tree species that use molecular tools is increasing, most of which collect leaf tissue for genomic DNA extraction. In tropical trees the canopy is not only frequently inaccessible, but also, once reached, the leaf tissue is often heavily defended against herbivory by high concentrations of anti-predation compounds, which may inhibit downstream applications, particularly PCR. Cambium tissue, accessed directly from the tree trunk at ground level, offers a readily accessible resource that is less hampered by the presence of defensive chemicals than leaf tissue. Here we describe a simple method for obtaining tissue from the cambial zone for DNA extraction and test the applicability of the method in a range of tropical tree species. The method was used successfully to extract DNA from 11 species in nine families. A subset of the DNA extracts was tested in more detail and proved to be highly suitable for AFLP analysis
dc.language.isoen
dc.publisherWalter De Gruyter
dc.subjectDNA EXTRACTION
dc.subjectAFLP
dc.subject.enTREES
dc.subject.enTROPICAL
dc.subject.enCAMBIUM
dc.subject.enSAMPLE PRESERVATION
dc.title.enSampling tissue for DNA analysis of trees: Trunk cambium as an alternative to canopy leaves
dc.typeArticle de revue
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles/Sylviculture, foresterie
bordeaux.journalSilvae Genetica
bordeaux.page265-269
bordeaux.volume54
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-02680896
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02680896v1
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