COLPAERT, N.; CAVERS, Stephen; BANDOU, Eric; CARON, Henri; GHEYSEN, Godelieve; LOWE, Johannes

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COLPAERT, N.
Universiteit Gent = Ghent University = Université de Gand [UGENT]

CAVERS, Stephen
Natural Environment Research Council [NERC]

BANDOU, Eric
Biodiversité, Gènes et Ecosystèmes [BioGeCo]

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Article de revue

Ce document a été publié dans

Silvae Genetica. 2005, vol. 54, n° 6, p. 265-269

Walter De Gruyter

Résumé en anglais

The number of studies of tropical tree species that use molecular tools is increasing, most of which collect leaf tissue for genomic DNA extraction. In tropical trees the canopy is not only frequently inaccessible, but also, once reached, the leaf tissue is often heavily defended against herbivory by high concentrations of anti-predation compounds, which may inhibit downstream applications, particularly PCR. Cambium tissue, accessed directly from the tree trunk at ground level, offers a readily accessible resource that is less hampered by the presence of defensive chemicals than leaf tissue. Here we describe a simple method for obtaining tissue from the cambial zone for DNA extraction and test the applicability of the method in a range of tropical tree species. The method was used successfully to extract DNA from 11 species in nine families. A subset of the DNA extracts was tested in more detail and proved to be highly suitable for AFLP analysis< Réduire

Mots clés

DNA EXTRACTION

AFLP

Mots clés en anglais

TREES

TROPICAL

CAMBIUM

SAMPLE PRESERVATION

Origine

Importé de hal

Unités de recherche

BioGeCo (Biodiversité Gènes & Communautés) - UMR 1202