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Annotation of the oak genome sequence and associated bioinformatic resources

hal.structure.identifierUnité de Recherche Génomique Info [URGI]
dc.contributor.authorAMSELEM, Joelle
hal.structure.identifierCommissariat à l'énergie atomique et aux énergies alternatives [CEA]
dc.contributor.authorAURY, Jean Marc
hal.structure.identifierEtude du Polymorphisme des Génomes Végétaux [EPGV]
dc.contributor.authorFRANCILLONNE, Nicolas
hal.structure.identifierEtude du Polymorphisme des Génomes Végétaux [EPGV]
dc.contributor.authorALAEITABAR, Tina
hal.structure.identifierCommissariat à l'énergie atomique et aux énergies alternatives [CEA]
dc.contributor.authorDA SILVA, Corinne
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorDUPLESSIS, Sébastien
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorEHRENMANN, François
hal.structure.identifierCommissariat à l'énergie atomique et aux énergies alternatives [CEA]
dc.contributor.authorFAYE, Sébastien
hal.structure.identifierUnité de Mathématiques et Informatique Appliquées de Toulouse [MIAT INRA]
dc.contributor.authorKLOPP, Christophe
hal.structure.identifierUnité de Mathématiques et Informatique Appliquées de Toulouse [MIAT INRA]
dc.contributor.authorGASPIN, Christine
hal.structure.identifierUnité de Mathématiques et Informatique Appliquées de Toulouse [MIAT INRA]
dc.contributor.authorRUË, Olivier
hal.structure.identifierCommissariat à l'énergie atomique et aux énergies alternatives [CEA]
dc.contributor.authorLABADIE, Karine
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLEROY, Thibault
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLESUR, Isabelle
hal.structure.identifierEtude du Polymorphisme des Génomes Végétaux [EPGV]
dc.contributor.authorFAIVRE-RAMPANT, Patricia
hal.structure.identifierUnité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]
dc.contributor.authorLEPLÉ, Jean-Charles
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorKREMER, Antoine
hal.structure.identifierInteractions Arbres-Microorganismes [IAM]
dc.contributor.authorMARTIN, Francis
hal.structure.identifierGénétique Diversité et Ecophysiologie des Céréales [GDEC]
dc.contributor.authorSALSE, Jerome
hal.structure.identifierUnité de Recherche Génomique Info [URGI]
dc.contributor.authorQUESNEVILLE, Hadi
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPLOMION, Christophe
dc.date.issued2016
dc.date.conference2016-05-30
dc.description.abstractEnThe large, complex and highly heterozygous genome of pedunculate oak (Quercus robur) was sequenced using a whole-genome shotgun approach [1] . Roche 454 GS-FLX sequence reads were assembled into contigs and combined with Illumina reads from paired-end, mate-pair libraries and true synthetic long reads to build a total of 8,827 scaffolds (1.46 Gb total size; N50=821 kb). Both haplotypes were merged into an haploid version and 12 pseudomolecules were established using a high-density linkage map [2] combined with a syntenome approach using the peach genome sequence.The structural (Transposable Elements (TEs), genes, ncRNA) and functional annotation of automatically predicted genes relies on powerful and robust pipelines: (i) REPET package [3] [4] was first used to de novo detect, classify and annotate TEs representing about 50% of the genome; (ii) Eugene was trained and launched to integrate ab initio and similarity gene finding software to finally predict 43,240 genes including 29,665 highly confident gene models; (iii) ncRNA were predicted using feelcn (lncRNA), similarities against databases and small RNAseq data analysis (miRNA), RNAmmer (rRNA), tRNAscan-SE (tRNA) and Infernal package (other non-coding RNA) (iv) A functional annotation pipeline mainly based on Interproscan to search for patterns/motifs and Blast based comparative genomics was launched onto the 43,240 predicted proteins. The assignation of a provisional definition for predicted protein according to the results of the most reliable tools and their occurrence in Oak annotation was produced (D. Goodstein method, personal communication). We will present here these pipelines and the results of this annotation.We also set up an integrated genome annotation system (dedicated to oak) based on GMOD web interfaces such as WebApollo/JBrowse and Intermine to make these data available under a user-friendly environment. This system allowed experts to analyze their respective protein families of interest and curate/validate gene structure. We will also present the interoperability between these genomic data and genetic data produced in Quercus (SNPs, linkage maps, QTLs) available in GnpIS [5] an information System for plants. All together these resources provide a framework to study the two key evolutionary processes that explain the remarkable diversity found within the Quercus genus: local adaptation and speciation.
dc.language.isoen
dc.title.enAnnotation of the oak genome sequence and associated bioinformatic resources
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halMathématiques [math]
dc.subject.halInformatique [cs]
dc.subject.halSciences de l'environnement
bordeaux.page134 p.
bordeaux.conference.titleIUFRO Genomics and Forest Tree Genetics
bordeaux.countryFR
bordeaux.conference.cityArcachon
bordeaux.peerReviewednon
hal.identifierhal-02741128
hal.version1
hal.invitednon
hal.conference.organizerInstitut National de Recherche Agronomique (INRA). UMR Biodiversité, Gènes et Communautés (1202).
hal.conference.end2016-06-03
hal.popularnon
hal.audienceNationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02741128v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2016&rft.spage=134%20p.&rft.epage=134%20p.&rft.au=AMSELEM,%20Joelle&AURY,%20Jean%20Marc&FRANCILLONNE,%20Nicolas&ALAEITABAR,%20Tina&DA%20SILVA,%20Corinne&rft.genre=unknown


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