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Sixty years from the first disease description, a novel badnavirus associated with chestnut mosaic disease

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
hal.structure.identifierPolytechnic University of Marche / Università Politecnica delle Marche [UNIVPM]
dc.contributor.authorMUROLO, Sergio
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFAURE, Chantal
hal.structure.identifierCentre Technique Interprofessionnel des Fruits et Légumes [CTIFL]
dc.contributor.authorBRANS, Yoann
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierInvenio
dc.contributor.authorLARUE, Clement
hal.structure.identifierUnité de recherche TERRA [Gembloux]
dc.contributor.authorMACLOT, François
hal.structure.identifierUnité de recherche TERRA [Gembloux]
dc.contributor.authorMASSART, Sébastien
hal.structure.identifierCNR Istituto per la Protezione Sostenibile delle Piante [Torino, Italia] [IPSP]
dc.contributor.authorCHIUMENTI, Michela
hal.structure.identifierCNR Istituto per la Protezione Sostenibile delle Piante [Torino, Italia] [IPSP]
dc.contributor.authorMINAFRA, Angelantonio
hal.structure.identifierPolytechnic University of Marche / Università Politecnica delle Marche [UNIVPM]
dc.contributor.authorROMANAZZI, Gianfranco
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLEFEBVRE, Marie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBARRENECHE, Teresa
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorROBIN, Cécile
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPETIT, Remy
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.issued2021
dc.identifier.issn0031-949X
dc.description.abstractEnAlthough chestnut mosaic disease (ChMD) was described several decades ago, its etiology is still not clear. Using classical approaches and high-throughput sequencing (HTS) techniques, we identified a novel Badnavirus that is a strong etiological candidate for ChMD. Two disease sources from Italy and France were submitted to HTS-based viral indexing. Total RNAs were extracted, ribodepleted, and sequenced on an Illumina NextSeq500 (2 × 150 nt or 2 × 75 nt). In each source, we identified a single contig of ≈7.2 kb that corresponds to a complete circular viral genome and shares homologies with various badnaviruses. The genomes of the two isolates have an average nucleotide identity of 90.5%, with a typical badnaviral genome organization comprising three open reading frames. Phylogenetic analyses and sequence comparisons showed that this virus is a novel species; we propose the name Chestnut mosaic virus (ChMV). Using a newly developed molecular detection test, we systematically detected the virus in symptomatic graft-inoculated indicator plants (chestnut and American oak) as well in chestnut trees presenting typical ChMD symptoms in the field (100 and 87% in France and Italy surveys, respectively). Datamining of publicly available chestnut sequence read archive transcriptomic data allowed the reconstruction of two additional complete ChMV genomes from two Castanea mollissima sources from the United States as well as ChMV detection in C. dentata from the United States. Preliminary epidemiological studies performed in France and central eastern Italy showed that ChMV has a high incidence in some commercial orchards and low within-orchard genetic diversity.
dc.language.isoen
dc.publisherAmerican Phytopathological Society
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enHigh throughput sequencing
dc.subject.enCastanea sp.
dc.subject.enetiology
dc.subject.enviral disease
dc.subject.enBadnavirus
dc.subject.enCaulimoviridae
dc.title.enSixty years from the first disease description, a novel badnavirus associated with chestnut mosaic disease
dc.typeArticle de revue
dc.identifier.doi10.1094/PHYTO-09-20-0420-R
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalPhytopathology
bordeaux.page1051-1058
bordeaux.volume111
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-03036205
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03036205v1
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