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Reversibly crosslinked thermo- and redox-responsive nanogels for controlled drug release

hal.structure.identifierCentre d'Etude et de Recherche sur les Macromolécules [CERM]
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorLIU, Ji
hal.structure.identifierCentre d'Etude et de Recherche sur les Macromolécules [CERM]
dc.contributor.authorDETREMBLEUR, Christophe
hal.structure.identifierCentre d'Etude et de Recherche sur les Macromolécules [CERM]
dc.contributor.authorHURTGEN, Marie
hal.structure.identifierCentre d'Etude et de Recherche sur les Macromolécules [CERM]
dc.contributor.authorDEBUIGNE, Antoine
hal.structure.identifierLaboratory of Mammalian Cell Culture [GIGA-R]
dc.contributor.authorDE PAUW-GILLET, Marie-Claire
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorDUGUET, Etienne
hal.structure.identifierCentre d'Etude et de Recherche sur les Macromolécules [CERM]
dc.contributor.authorJÉRÔME, Christine
dc.date.issued2014
dc.identifier.issn1759-9954
dc.description.abstractEnReversibly crosslinked poly(vinyl alcohol)-b-poly(N-vinylcaprolactam) PVOH-b-PNVCL nanogels were prepared by using a redox-responsive crosslinking agent, 3,3′-dithiodipropionic acid (DPA), to crosslink the PVOH corona, above the lower critical solution temperature (LCST) of the PNVCL block. The stability of the as-prepared nanogels against heating and diluting with water was studied by dynamic light scattering (DLS) to follow the evolution of the hydrodynamic diameter and size distribution. Stability under reductive conditions was also studied by DLS and transmission electron microscopy (TEM) after exposure to dithiothreitol (DTT) buffer solutions at different pH. The reversibility of the crosslinking was evaluated by treating the de-crosslinked nanogels with hydrogen peroxide (H2O2) above the LCST. As a hydrophobic drug model, Nile red (NR) was loaded into the nanogels, and triggered release behaviours were studied after exposure to the same DTT buffer solutions. Moreover, two PVOH-b-PNVCL copolymers with different compositions and LCST were used to evaluate the effect of the LCST on the release behaviours of the nanogels. The cytotoxicity of the nanogels against a mouse fibroblast-like L929 cell line was assessed via the MTS assay, and preliminary studies on cellular uptake of the nanogels within human melanoma MEL-5 cells were also carried out by fluorescence microscopy and fluorescence-activated cell sorting.
dc.language.isoen
dc.publisherRoyal Society of Chemistry - RSC
dc.title.enReversibly crosslinked thermo- and redox-responsive nanogels for controlled drug release
dc.typeArticle de revue
dc.identifier.doi10.1039/c3py00839h
dc.subject.halChimie/Matériaux
bordeaux.journalPolymer Chemistry
bordeaux.page77-88
bordeaux.volume5
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-00923642
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00923642v1
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